Please use this identifier to cite or link to this item:
doi:10.22028/D291-35457
Title: | Active Janus Droplet as a Micro‐Reactor for Automatic DNA/RNA Precipitation and Extraction |
Author(s): | Li, Menglin Seemann, Ralf Fleury, Jean-Baptiste |
Language: | English |
Title: | ChemistrySelect |
Volume: | 6 |
Issue: | 15 |
Pages: | 3722–372 |
Publisher/Platform: | Wiley |
Year of Publication: | 2021 |
Free key words: | Saarland University Experimental physics and Center for Biophysics Saarbruecken Germany |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | We explore the possibility to precipitate and to extract DNA, or RNA using an active water/ethanol Janus droplet. The active Janus droplet is initially formed by a single droplet made of a water/ethanol mixture in an oil/surfactant solution. This active droplet self-propels and absorbs surfactant molecules, by advection, while moving in the oily phase. The surfactant absorption leads to a phase separation of the water/ethanol mixture and the formation of an active Janus droplet. This active Janus droplet is made of a water-rich leading droplet and an ethanol-rich trailing droplet. We employ this phase separation method to precipitate and to extract DNA, RNA, or DNA/RNA mixtures independently, in an automatic manner. This automatic precipitation and extraction of DNA, or RNA, is achieved by tuning their water solubility with the addition of salt and then it is demonstrated by fluorescence. |
DOI of the first publication: | 10.1002/slct.202003940 |
Link to this record: | urn:nbn:de:bsz:291--ds-354572 hdl:20.500.11880/32378 http://dx.doi.org/10.22028/D291-35457 |
ISSN: | 2365-6549 |
Date of registration: | 9-Feb-2022 |
Description of the related object: | Supporting Information |
Related object: | https://chemistry-europe.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fslct.202003940&file=slct202003940-sup-0001-movie1.avi |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Physik |
Professorship: | NT - Prof. Dr. Ralf Seemann |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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ChemistrySelect - 2021 - Li - Active Janus Droplet as a Micro‐Reactor for Automatic DNA RNA Precipitation and Extraction.pdf | 1,45 MB | Adobe PDF | View/Open |
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