Please use this identifier to cite or link to this item: doi:10.22028/D291-35457
Title: Active Janus Droplet as a Micro‐Reactor for Automatic DNA/RNA Precipitation and Extraction
Author(s): Li, Menglin
Seemann, Ralf
Fleury, Jean‐Baptiste
Language: English
Title: ChemistrySelect
Volume: 6
Issue: 15
Pages: 3722–372
Publisher/Platform: Wiley
Year of Publication: 2021
Free key words: Saarland University
Experimental physics and Center for Biophysics
Saarbruecken
Germany
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: We explore the possibility to precipitate and to extract DNA, or RNA using an active water/ethanol Janus droplet. The active Janus droplet is initially formed by a single droplet made of a water/ethanol mixture in an oil/surfactant solution. This active droplet self-propels and absorbs surfactant molecules, by advection, while moving in the oily phase. The surfactant absorption leads to a phase separation of the water/ethanol mixture and the formation of an active Janus droplet. This active Janus droplet is made of a water-rich leading droplet and an ethanol-rich trailing droplet. We employ this phase separation method to precipitate and to extract DNA, RNA, or DNA/RNA mixtures independently, in an automatic manner. This automatic precipitation and extraction of DNA, or RNA, is achieved by tuning their water solubility with the addition of salt and then it is demonstrated by fluorescence.
DOI of the first publication: 10.1002/slct.202003940
Link to this record: urn:nbn:de:bsz:291--ds-354572
hdl:20.500.11880/32378
http://dx.doi.org/10.22028/D291-35457
ISSN: 2365-6549
Date of registration: 9-Feb-2022
Description of the related object: Supporting Information
Related object: https://chemistry-europe.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fslct.202003940&file=slct202003940-sup-0001-movie1.avi
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Ralf Seemann
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



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