Please use this identifier to cite or link to this item: doi:10.22028/D291-33926
Title: Acanthocyte Sedimentation Rate as a Diagnostic Biomarker for Neuroacanthocytosis Syndromes: Experimental Evidence and Physical Justification
Author(s): Darras, Alexis
Peikert, Kevin
Rabe, Antonia
Yaya, François
Simionato, Greta
John, Thomas
Dasanna, Anil Kumar
Buvalyy, Semen
Geisel, Jürgen
Hermann, Andreas
Fedosov, Dmitry A.
Danek, Adrian
Wagner, Christian
Kaestner, Lars
Language: English
Title: Cells
Volume: 10
Issue: 4
Publisher/Platform: MDPI
Year of Publication: 2021
Free key words: neuroacanthocytosis
erythrocyte sedimentation rate (ESR)
diagnosis
DDC notations: 500 Science
530 Physics
610 Medicine and health
Publikation type: Journal Article
Abstract: (1) Background: Chorea-acanthocytosis and McLeod syndrome are the core diseases among the group of rare neurodegenerative disorders called neuroacanthocytosis syndromes (NASs). NAS patients have a variable number of irregularly spiky erythrocytes, so-called acanthocytes. Their detection is a crucial but error-prone parameter in the diagnosis of NASs, often leading to misdiagnoses. (2) Methods: We measured the standard Westergren erythrocyte sedimentation rate (ESR) of various blood samples from NAS patients and healthy controls. Furthermore, we manipulated the ESR by swapping the erythrocytes and plasma of different individuals, as well as replacing plasma with dextran. These measurements were complemented by clinical laboratory data and single-cell adhesion force measurements. Additionally, we followed theoretical modeling approaches. (3) Results: We show that the acanthocyte sedimentation rate (ASR) with a two-hour read-out is significantly prolonged in chorea-acanthocytosis and McLeod syndrome without overlap compared to the ESR of the controls. Mechanistically, through modern colloidal physics, we show that acanthocyte aggregation and plasma fibrinogen levels slow down the sedimentation. Moreover, the inverse of ASR correlates with the number of acanthocytes (R 2 = 0.61, p = 0.004). (4) Conclusions: The ASR/ESR is a clear, robust and easily obtainable diagnostic marker. Independently of NASs, we also regard this study as a hallmark of the physical view of erythrocyte sedimentation by describing anticoagulated blood in stasis as a percolating gel, allowing the application of colloidal physics theory.
DOI of the first publication: 10.3390/cells10040788
Link to this record: urn:nbn:de:bsz:291--ds-339264
hdl:20.500.11880/31319
http://dx.doi.org/10.22028/D291-33926
ISSN: 2073-4409
Date of registration: 17-May-2021
Description of the related object: Supplementary Materials
Related object: https://www.mdpi.com/2073-4409/10/4/788/s1
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Anatomie und Zellbiologie
M - Innere Medizin
NT - Physik
Professorship: M - Keiner Professur zugeordnet
NT - Prof. Dr. Christian Wagner
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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