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doi:10.22028/D291-44499 | Title: | A protocol for CRISPR-mediated activation and repression of human endogenous retroviruses in human pluripotent stem cells |
| Author(s): | Nair, Vidya Padmanabhan Mayer, Jens Vincendeau, Michelle |
| Language: | English |
| Title: | STAR Protocols |
| Volume: | 3 |
| Issue: | 2 |
| Publisher/Platform: | Elsevier |
| Year of Publication: | 2022 |
| DDC notations: | 610 Medicine and health |
| Publikation type: | Journal Article |
| Abstract: | Human endogenous retroviruses (HERVs) comprise many regulatory elements and can regulate host gene activity at different expression levels via multiple mechanisms. Here, we introduce a step-by-step protocol to activate or repress transcription of HERV-K(HML-2) elements using the CRISPRa and CRISPRi technologies in human embryonic stem cells. This protocol can help deciphering the functional role of HERV-K(HML-2) elements in critical biological processes. The protocol may easily be adapted to other cell lines and HERV groups with relatively low sequence heterogeneity. For complete details on the use and execution of this protocol, please refer to Padmanabhan Nair et al. (2021). |
| DOI of the first publication: | 10.1016/j.xpro.2022.101281 |
| URL of the first publication: | https://www.sciencedirect.com/science/article/pii/S2666166722001617 |
| Link to this record: | urn:nbn:de:bsz:291--ds-444998 hdl:20.500.11880/39754 http://dx.doi.org/10.22028/D291-44499 |
| ISSN: | 2666-1667 |
| Date of registration: | 5-Mar-2025 |
| Faculty: | M - Medizinische Fakultät |
| Department: | M - Humangenetik |
| Professorship: | M - Prof. Dr. Eckart Meese |
| Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 1-s2.0-S2666166722001617-main.pdf | 3,57 MB | Adobe PDF | View/Open |
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