Please use this identifier to cite or link to this item: doi:10.22028/D291-41612
Title: The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells
Author(s): Petkova-Kirova, Polina
Murciano, Nicoletta
Iacono, Giulia
Jansen, Julia
Simionato, Greta
Qiao, Min
Van der Zwaan, Carmen
Rotordam, Maria Giustina
John, Thomas
Hertz, Laura
Hoogendijk, Arjan J.
Becker, Nadine
Wagner, Christian
Von Lindern, Marieke
Egee, Stephane
Van den Akker, Emile
Kaestner, Lars
Language: English
Title: International Journal of Molecular Sciences
Volume: 25
Issue: 3
Publisher/Platform: MDPI
Year of Publication: 2024
Free key words: erythrocytes
patch clamp
membrane potential
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in the red blood cell (RBC) membrane. Nevertheless, the quantitative electrophysiological behavior of RBCs and its heterogeneity are still not completely understood. Here, we use state-of-the-art biochemical methods to probe for the abundance of the channels in RBCs. Furthermore, we utilize automated patch clamp, based on planar chips, to compare the activity of the two channels in reticulocytes and mature RBCs. In addition to this characterization, we performed membrane potential measurements to demonstrate the effect of channel activity and interplay on the RBC properties. Both the Gárdos channel and Piezo1, albeit their average copy number of activatable channels per cell is in the single-digit range, can be detected through transcriptome analysis of reticulocytes. Proteomics analysis of reticulocytes and mature RBCs could only detect Piezo1 but not the Gárdos channel. Furthermore, they can be reliably measured in the whole-cell configuration of the patch clamp method. While for the Gárdos channel, the activity in terms of ion currents is higher in reticulocytes compared to mature RBCs, for Piezo1, the tendency is the opposite. While the interplay between Piezo1 and Gárdos channel cannot be followed using the patch clamp measurements, it could be proved based on membrane potential measurements in populations of intact RBCs. We discuss the Gárdos channel and Piezo1 abundance, interdependencies and interactions in the context of their proposed physiological and pathophysiological functions, which are the passing of small constrictions, e.g., in the spleen, and their active participation in blood clot formation and thrombosis.
DOI of the first publication: 10.3390/ijms25031416
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-416121
ISSN: 1422-0067
Date of registration: 13-Feb-2024
Faculty: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Department: M - Anatomie und Zellbiologie
NT - Physik
Professorship: M - Keiner Professur zugeordnet
NT - Prof. Dr. Christian Wagner
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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