Please use this identifier to cite or link to this item: doi:10.22028/D291-41539
Title: Dual Sec62/Ki67 immunocytochemistry of liquid-based cytological preparations represents a highly valid biomarker for non-invasive detection of head and neck squamous cell carcinomas
Author(s): Kühn, Jan Philipp
Speicher, Stefanie
Linxweiler, Barbara
Körner, Sandrina
Rimbach, Hugo
Wagner, Mathias
Solomayer, Erich Franz
Schick, Bernhard
Linxweiler, Maximilian
Language: English
Title: Cytopathology
Volume: 35 (2024)
Issue: 1
Pages: 113-121
Publisher/Platform: Wiley
Year of Publication: 2023
Free key words: diagnostic biomarkers
head and neck squamous cell carcinoma
immunocytochemistry
Ki67
liquid-based cytology
Sec62
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Background: Head and neck squamous cell carcinomas (HNSCC) are frequently diagnosed in advanced stages, which limits therapeutic options and results in persistently poor patient outcomes. The aim of this study was to use liquid-based swab cytology (LBC) in combination with dual immunocytochemical detection of migration and proliferation markers Sec62 and Ki67 in order to allow non-invasive early detection of HNSCC as well as to analyse the diagnostic validity of this method for predicting the malignancy of suspicious oral lesions. Methods: 104 HNSCC patients and 28 control patients, including healthy patients (n= 17), papilloma (n= 1) and leukoplakia patients (n= 10), were included in this study. For all patients, an LBC swab followed by simultaneous immunocytochemical detection of Sec62 and Ki67 was performed. Immunocytochemical as well as cytopathological results were correlated with histological diagnoses and clinical findings. Results: All HNSCC patients (100%) showed dual Sec62/Ki67 positivity, and all control patients except for the papilloma patient were negative for Sec62/Ki67 (96.4%), resulting in a 100% sensitivity and 96.4% specificity of Sec62/Ki67 dual stain for noninvasive detection of HNSCC. The positive predictive value was 99% and the negative predictive value was 100%. Sec62 expression levels showed a positive correlation with tumour de-differentiation (p= 0.0489). Conclusion: Simultaneous immunocytochemical detection of Sec62/Ki67 using LBC represents a promising non-invasive and easy-to-apply tool for the early detection of HNSCC in routine clinical practice. This novel technique can help to avoid incisional biopsies and reduce the frequency with which general anaesthesia is used in diagnostic procedures in patients with suspicious oral lesions.
DOI of the first publication: 10.1111/cyt.13310
URL of the first publication: https://onlinelibrary.wiley.com/doi/10.1111/cyt.13310
Link to this record: urn:nbn:de:bsz:291--ds-415393
hdl:20.500.11880/37220
http://dx.doi.org/10.22028/D291-41539
ISSN: 1365-2303
Date of registration: 1-Feb-2024
Faculty: M - Medizinische Fakultät
Department: M - Frauenheilkunde
M - Hals-Nasen-Ohrenheilkunde
M - Pathologie
Professorship: M - Prof. Dr. Bernhard Schick
M - Prof. Dr. E.-F. Solomayer
M - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



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