Please use this identifier to cite or link to this item: doi:10.22028/D291-41297
Title: Carriage of linezolid-resistant enterococci (LRE) among humans and animals in Nigeria: coexistence of the cfr, optrA, and poxtA genes in Enterococcus faecium of animal origin
Author(s): Ngbede, Emmanuel O.
Sy, Issa
Akwuobu, Chinedu A.
Nanven, Maurice A.
Adikwu, Alex A.
Abba, Paul O.
Adah, Mohammed I.
Becker, Sören L.
Language: English
Title: Journal of Global Antimicrobial Resistance
Volume: 34
Pages: 234-239
Publisher/Platform: Elsevier
Year of Publication: 2023
Free key words: Antimicrobial resistance
Linezolid-resistant enterococci
cfr
oprtA
poxtA
Nigeria
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Objectives: In contrast to increasing reports of the emergence of linezolid-resistant enterococci (LRE) emanating from many countries in Europe, Asia, and North America, data on its status and dissemination from the African continent remain scarce, with the information available limited to countries in North Africa. This study investigated the carriage of LRE and the genetic mechanism of resistance among Enterococcus faecium and Enterococcus faecalis strains recovered from humans and animals in Makurdi, Nigeria. Methods: We conducted a cross-sectional study between June 2020 and July 2021 during which 630 nonduplicate human and animal faecal samples were collected and processed for the recovery of LRE. The genetic mechanisms for resistance were investigated using polymerase chain reaction (PCR) and Sanger sequencing. Results: Linezolid-resistant enterococci were recovered from 5.87% (37/630; 95% CI: 4.17–8.00) of the samples, with the prevalence in animals and humans being 6.22% [(28/450); 95% CI: 4.17–8.87] and 5.00% [(9/180); 95% CI: 2.31–9.28], respectively. All isolates remained susceptible to vancomycin. No known point mutation mediating linezolid resistance was detected in the 23S rRNA and ribosomal protein genes; however, acquisition of one or more potentially transferable genes (cfr, optrA, and poxtA) was observed in 26 of the 37 LRE isolates. Co-existence of all three transferable genes in a single isolate was found in four E. faecium strains of animal origin. Conclusion: This study provides baseline evidence for the emergence and active circulation of LRE driven majorly by the acquisition of the optrA gene in Nigeria. To the best of our knowledge, our study is the first to report a co-carriage of all three transferable linezolid resistance determinants in E. faecium. Active LRE surveillance is urgently required to understand the extent of LRE spread across sub-Saharan Africa and to develop tailored mitigation strategies.
DOI of the first publication: 10.1016/j.jgar.2023.07.016
URL of the first publication: https://doi.org/10.1016/j.jgar.2023.07.016
Link to this record: urn:nbn:de:bsz:291--ds-412978
hdl:20.500.11880/37038
http://dx.doi.org/10.22028/D291-41297
ISSN: 2213-7165
Date of registration: 12-Dec-2023
Description of the related object: Supplementary materials
Related object: https://ars.els-cdn.com/content/image/1-s2.0-S2213716523001200-mmc1.docx
Faculty: M - Medizinische Fakultät
Department: M - Infektionsmedizin
Professorship: M - Prof. Dr. Sören Becker
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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