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doi:10.22028/D291-40058
Titel: | Paralogs of the Calcium-Dependent Activator Protein for Secretion Differentially Regulate Synaptic Transmission and Peptide Secretion in Sensory Neurons |
VerfasserIn: | Shaib, Ali H. Staudt, Angelina Harb, Ali Klose, Margarete Shaaban, Ahmed Schirra, Claudia Mohrmann, Ralf Rettig, Jens Becherer, Ute |
Sprache: | Englisch |
Titel: | Frontiers in Cellular Neuroscience |
Bandnummer: | 12 |
Verlag/Plattform: | Frontiers |
Erscheinungsjahr: | 2018 |
Freie Schlagwörter: | DRG neurons CADPS exocytosis priming large dense core vesicle synaptic transmission neuropeptide |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | The two paralogs of the calcium-dependent activator protein for secretion (CAPS) are priming factors for synaptic vesicles (SVs) and neuropeptide containing large dense-core vesicles (LDCVs). Yet, it is unclear whether CAPS1 and CAPS2 regulate exocytosis of these two vesicle types differentially in dorsal root ganglion (DRG) neurons, wherein synaptic transmission and neuropeptide release are of equal importance. These sensory neurons transfer information from the periphery to the spinal cord (SC), releasing glutamate as the primary neurotransmitter, with co-transmission via neuropeptides in a subset of so called peptidergic neurons. Neuropeptides are key components of the information-processing machinery of pain perception and neuropathic pain generation. Here, we compared the ability of CAPS1 and CAPS2 to support priming of both vesicle types in single and double knock-out mouse (DRG) neurons using a variety of high-resolution live cell imaging methods. While CAPS1 was localized to synapses of all DRG neurons and promoted synaptic transmission, CAPS2 was found exclusively in peptidergic neurons and mediated LDCV exocytosis. Intriguingly, ectopic expression of CAPS2 empowered non-peptidergic neurons to drive LDCV fusion, thereby identifying CAPS2 as an essential molecular determinant for peptidergic signaling. Our results reveal that these distinct functions of both CAPS paralogs are based on their differential subcellular localization in DRG neurons. Our data suggest a major role for CAPS2 in neuropathic pain via control of neuropeptide release. |
DOI der Erstveröffentlichung: | 10.3389/fncel.2018.00304 |
URL der Erstveröffentlichung: | https://www.frontiersin.org/articles/10.3389/fncel.2018.00304 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-400584 hdl:20.500.11880/36067 http://dx.doi.org/10.22028/D291-40058 |
ISSN: | 1662-5102 |
Datum des Eintrags: | 3-Jul-2023 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Material |
In Beziehung stehendes Objekt: | https://www.frontiersin.org/articles/file/downloadfile/410078_supplementary-materials_datasheets_1_pdf/octet-stream/Data%20Sheet%201.pdf/2/410078 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Physiologie |
Professur: | M - Prof. Dr. Jens Rettig |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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fncel-12-00304.pdf | 3,76 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons