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Titel: Paralogs of the Calcium-Dependent Activator Protein for Secretion Differentially Regulate Synaptic Transmission and Peptide Secretion in Sensory Neurons
VerfasserIn: Shaib, Ali H.
Staudt, Angelina
Harb, Ali
Klose, Margarete
Shaaban, Ahmed
Schirra, Claudia
Mohrmann, Ralf
Rettig, Jens
Becherer, Ute
Sprache: Englisch
Titel: Frontiers in Cellular Neuroscience
Bandnummer: 12
Verlag/Plattform: Frontiers
Erscheinungsjahr: 2018
Freie Schlagwörter: DRG neurons
CADPS
exocytosis
priming
large dense core vesicle
synaptic transmission
neuropeptide
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The two paralogs of the calcium-dependent activator protein for secretion (CAPS) are priming factors for synaptic vesicles (SVs) and neuropeptide containing large dense-core vesicles (LDCVs). Yet, it is unclear whether CAPS1 and CAPS2 regulate exocytosis of these two vesicle types differentially in dorsal root ganglion (DRG) neurons, wherein synaptic transmission and neuropeptide release are of equal importance. These sensory neurons transfer information from the periphery to the spinal cord (SC), releasing glutamate as the primary neurotransmitter, with co-transmission via neuropeptides in a subset of so called peptidergic neurons. Neuropeptides are key components of the information-processing machinery of pain perception and neuropathic pain generation. Here, we compared the ability of CAPS1 and CAPS2 to support priming of both vesicle types in single and double knock-out mouse (DRG) neurons using a variety of high-resolution live cell imaging methods. While CAPS1 was localized to synapses of all DRG neurons and promoted synaptic transmission, CAPS2 was found exclusively in peptidergic neurons and mediated LDCV exocytosis. Intriguingly, ectopic expression of CAPS2 empowered non-peptidergic neurons to drive LDCV fusion, thereby identifying CAPS2 as an essential molecular determinant for peptidergic signaling. Our results reveal that these distinct functions of both CAPS paralogs are based on their differential subcellular localization in DRG neurons. Our data suggest a major role for CAPS2 in neuropathic pain via control of neuropeptide release.
DOI der Erstveröffentlichung: 10.3389/fncel.2018.00304
URL der Erstveröffentlichung: https://www.frontiersin.org/articles/10.3389/fncel.2018.00304
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-400584
hdl:20.500.11880/36067
http://dx.doi.org/10.22028/D291-40058
ISSN: 1662-5102
Datum des Eintrags: 3-Jul-2023
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Material
In Beziehung stehendes Objekt: https://www.frontiersin.org/articles/file/downloadfile/410078_supplementary-materials_datasheets_1_pdf/octet-stream/Data%20Sheet%201.pdf/2/410078
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Physiologie
Professur: M - Prof. Dr. Jens Rettig
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons