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doi:10.22028/D291-38918
Title: | Antibacterial Action of Nanoparticles by Lethal Stretching of Bacterial Cell Membranes |
Author(s): | Linklater, Denver P. Baulin, Vladimir A. Le Guével, Xavier Fleury, Jean-Baptiste Hanssen, Eric Nguyen, The Hong Phong Juodkazis, Saulius Bryant, Gary Crawford, Russell J. Stoodley, Paul Ivanova, Elena P. |
Language: | English |
Title: | Advanced Materials |
Volume: | 32 |
Issue: | 52 |
Publisher/Platform: | Wiley |
Year of Publication: | 2020 |
Free key words: | mechano-bactericidal activity nanoparticles nanotoxicity |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | It is commonly accepted that nanoparticles (NPs) can kill bacteria; however, the mechanism of antimicrobial action remains obscure for large NPs that cannot translocate the bacterial cell wall. It is demonstrated that the increase in membrane tension caused by the adsorption of NPs is responsible for mechanical deformation, leading to cell rupture and death. A biophysical model of the NP–membrane interactions is presented which suggests that adsorbed NPs cause membrane stretching and squeezing. This general phenomenon is demonstrated experimentally using both model membranes and Pseudomonas aeruginosa and Staphylococcus aureus, representing Gram-positive and Gram-negative bacteria. Hydrophilic and hydrophobic quasi-spherical and star-shaped gold (Au)NPs are synthesized to explore the antibacterial mechanism of non-translocating AuNPs. Direct observation of nanoparticle-induced membrane tension and squeezing is demonstrated using a custom-designed microfluidic device, which relieves contraction of the model membrane surface area and eventual lipid bilayer collapse. Quasi-spherical nanoparticles exhibit a greater bactericidal action due to a higher interactive affinity, resulting in greater membrane stretching and rupturing, corroborating the theoretical model. Electron microscopy techniques are used to characterize the NP–bacterial-membrane interactions. This combination of experimental and theoretical results confirm the proposed mechanism of membrane-tension-induced (mechanical) killing of bacterial cells by non-translocating NPs. |
DOI of the first publication: | 10.1002/adma.202005679 |
URL of the first publication: | https://doi.org/10.1002/adma.202005679 |
Link to this record: | urn:nbn:de:bsz:291--ds-389181 hdl:20.500.11880/35110 http://dx.doi.org/10.22028/D291-38918 |
ISSN: | 1521-4095 0935-9648 |
Date of registration: | 6-Feb-2023 |
Description of the related object: | Supporting Information |
Related object: | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadma.202005679&file=adma202005679-sup-0001-SuppMat.pdf https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadma.202005679&file=adma202005679-sup-0002-MovieS1.avi https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadma.202005679&file=adma202005679-sup-0003-MovieS2.mp4 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Physik |
Professorship: | NT - Prof. Dr. Ralf Seemann |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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