Please use this identifier to cite or link to this item: doi:10.22028/D291-38918
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Title: Antibacterial Action of Nanoparticles by Lethal Stretching of Bacterial Cell Membranes
Author(s): Linklater, Denver P.
Baulin, Vladimir A.
Le Guével, Xavier
Fleury, Jean-Baptiste
Hanssen, Eric
Nguyen, The Hong Phong
Juodkazis, Saulius
Bryant, Gary
Crawford, Russell J.
Stoodley, Paul
Ivanova, Elena P.
Language: English
Title: Advanced Materials
Volume: 32
Issue: 52
Publisher/Platform: Wiley
Year of Publication: 2020
Free key words: mechano-bactericidal activity
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: It is commonly accepted that nanoparticles (NPs) can kill bacteria; however, the mechanism of antimicrobial action remains obscure for large NPs that cannot translocate the bacterial cell wall. It is demonstrated that the increase in membrane tension caused by the adsorption of NPs is responsible for mechanical deformation, leading to cell rupture and death. A biophysical model of the NP–membrane interactions is presented which suggests that adsorbed NPs cause membrane stretching and squeezing. This general phenomenon is demonstrated experimentally using both model membranes and Pseudomonas aeruginosa and Staphylococcus aureus, representing Gram-positive and Gram-negative bacteria. Hydrophilic and hydrophobic quasi-spherical and star-shaped gold (Au)NPs are synthesized to explore the antibacterial mechanism of non-translocating AuNPs. Direct observation of nanoparticle-induced membrane tension and squeezing is demonstrated using a custom-designed microfluidic device, which relieves contraction of the model membrane surface area and eventual lipid bilayer collapse. Quasi-spherical nanoparticles exhibit a greater bactericidal action due to a higher interactive affinity, resulting in greater membrane stretching and rupturing, corroborating the theoretical model. Electron microscopy techniques are used to characterize the NP–bacterial-membrane interactions. This combination of experimental and theoretical results confirm the proposed mechanism of membrane-tension-induced (mechanical) killing of bacterial cells by non-translocating NPs.
DOI of the first publication: 10.1002/adma.202005679
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-389181
ISSN: 1521-4095
Date of registration: 6-Feb-2023
Description of the related object: Supporting Information
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Ralf Seemann
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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