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doi:10.22028/D291-38498
Titel: | Differentiation potential and functional properties of a CD34‑CD133+ subpopulation of endothelial progenitor cells |
VerfasserIn: | Bachelier, Katrin Bergholz, Carolin Friedrich, Erik B. |
Sprache: | Englisch |
Titel: | Molecular Medicine Reports |
Bandnummer: | 21 (2020) |
Heft: | 1 |
Seiten: | 501-507 |
Verlag/Plattform: | Spandidos Publications |
Erscheinungsjahr: | 2019 |
Freie Schlagwörter: | endothelial progenitor cell subpopulations pluripotency adhesion migration proliferation differentiation |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Endothelial progenitor cells (EPCs) promote angiogenesis and play an important role in myocardial and vascular repair after ischemia and infarction. EPCs consist of different subpopulations including CD34- CD133+ EPCs, which are precursors of more mature CD34+CD133+ EPCs and functionally more active in terms of homing and endothelial regeneration. In the present study we analyzed the functional and differentiation abilities of CD34- CD133+ EPCs. Isolation of EPC populations (CD34+CD133+ , CD34- CD133+ ) were performed by specific multi-step magnetic depletion. After specific stimulation a significant higher adhesive and migrative capacity of CD34- CD133+ cells could be detected compared to CD34+CD133+ cells (P<0.001, respectively). Next to this finding, not only significantly higher rates of proliferation (P<0.005) were detected among CD34- CD133+ cells, but also a higher potential of cell-differentiation capacity into other cell types. Next to a significant increase of CD34- CD133+ EPCs differentiating into a fibroblast cell‑type (P<0.001), an enhancement into a hepatocytic cell-type (P=0.033) and a neural cell-type (P=0.016) could be measured in contrast to CD34+CD133+ cells. On the other hand, there was no significant difference in differentiation into a cardiomyocyte cell-type between these EPC subpopulations (P=0.053). These results demonstrate that EPC subpopulations vary in their functional abilities and, to different degrees, have the capacity to transdifferentiate into unrelated cell-types such as fibroblasts, hepatocytes, and neurocytes. This indicates that CD34- CD133+ cells are more pluripotent compared to the CD34+CD133+ EPC subset, which may have important consequences for the therapy of vascular diseases. |
DOI der Erstveröffentlichung: | 10.3892/mmr.2019.10831 |
URL der Erstveröffentlichung: | http://dx.doi.org/10.3892/mmr.2019.10831 |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-384987 hdl:20.500.11880/34717 http://dx.doi.org/10.22028/D291-38498 |
ISSN: | 1791-3004 1791-2997 |
Datum des Eintrags: | 9-Dez-2022 |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Innere Medizin |
Professur: | M - Keiner Professur zugeordnet |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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