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|Title:||Differentiation potential and functional properties of a CD34‑CD133+ subpopulation of endothelial progenitor cells|
Friedrich, Erik B.
|Title:||Molecular Medicine Reports|
|Year of Publication:||2019|
|Free key words:||endothelial progenitor cell subpopulations|
|DDC notations:||610 Medicine and health|
|Publikation type:||Journal Article|
|Abstract:||Endothelial progenitor cells (EPCs) promote angiogenesis and play an important role in myocardial and vascular repair after ischemia and infarction. EPCs consist of different subpopulations including CD34- CD133+ EPCs, which are precursors of more mature CD34+CD133+ EPCs and functionally more active in terms of homing and endothelial regeneration. In the present study we analyzed the functional and differentiation abilities of CD34- CD133+ EPCs. Isolation of EPC populations (CD34+CD133+ , CD34- CD133+ ) were performed by specific multi-step magnetic depletion. After specific stimulation a significant higher adhesive and migrative capacity of CD34- CD133+ cells could be detected compared to CD34+CD133+ cells (P<0.001, respectively). Next to this finding, not only significantly higher rates of proliferation (P<0.005) were detected among CD34- CD133+ cells, but also a higher potential of cell-differentiation capacity into other cell types. Next to a significant increase of CD34- CD133+ EPCs differentiating into a fibroblast cell‑type (P<0.001), an enhancement into a hepatocytic cell-type (P=0.033) and a neural cell-type (P=0.016) could be measured in contrast to CD34+CD133+ cells. On the other hand, there was no significant difference in differentiation into a cardiomyocyte cell-type between these EPC subpopulations (P=0.053). These results demonstrate that EPC subpopulations vary in their functional abilities and, to different degrees, have the capacity to transdifferentiate into unrelated cell-types such as fibroblasts, hepatocytes, and neurocytes. This indicates that CD34- CD133+ cells are more pluripotent compared to the CD34+CD133+ EPC subset, which may have important consequences for the therapy of vascular diseases.|
|DOI of the first publication:||10.3892/mmr.2019.10831|
|URL of the first publication:||http://dx.doi.org/10.3892/mmr.2019.10831|
|Link to this record:||urn:nbn:de:bsz:291--ds-384987|
|Date of registration:||9-Dec-2022|
|Faculty:||M - Medizinische Fakultät|
|Department:||M - Innere Medizin|
|Professorship:||M - Keiner Professur zugeordnet|
|Collections:||SciDok - Der Wissenschaftsserver der Universität des Saarlandes|
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