Please use this identifier to cite or link to this item: doi:10.22028/D291-38498
Volltext verfügbar? / Dokumentlieferung
Title: Differentiation potential and functional properties of a CD34‑CD133+ subpopulation of endothelial progenitor cells
Author(s): Bachelier, Katrin
Bergholz, Carolin
Friedrich, Erik B.
Language: English
Title: Molecular Medicine Reports
Volume: 21 (2020)
Issue: 1
Pages: 501-507
Publisher/Platform: Spandidos Publications
Year of Publication: 2019
Free key words: endothelial progenitor cell subpopulations
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Endothelial progenitor cells (EPCs) promote angiogenesis and play an important role in myocardial and vascular repair after ischemia and infarction. EPCs consist of different subpopulations including CD34- CD133+ EPCs, which are precursors of more mature CD34+CD133+ EPCs and functionally more active in terms of homing and endothelial regeneration. In the present study we analyzed the functional and differentiation abilities of CD34- CD133+ EPCs. Isolation of EPC populations (CD34+CD133+ , CD34- CD133+ ) were performed by specific multi-step magnetic depletion. After specific stimulation a significant higher adhesive and migrative capacity of CD34- CD133+ cells could be detected compared to CD34+CD133+ cells (P<0.001, respectively). Next to this finding, not only significantly higher rates of proliferation (P<0.005) were detected among CD34- CD133+ cells, but also a higher potential of cell-differentiation capacity into other cell types. Next to a significant increase of CD34- CD133+ EPCs differentiating into a fibroblast cell‑type (P<0.001), an enhancement into a hepatocytic cell-type (P=0.033) and a neural cell-type (P=0.016) could be measured in contrast to CD34+CD133+ cells. On the other hand, there was no significant difference in differentiation into a cardiomyocyte cell-type between these EPC subpopulations (P=0.053). These results demonstrate that EPC subpopulations vary in their functional abilities and, to different degrees, have the capacity to transdifferentiate into unrelated cell-types such as fibroblasts, hepatocytes, and neurocytes. This indicates that CD34- CD133+ cells are more pluripotent compared to the CD34+CD133+ EPC subset, which may have important consequences for the therapy of vascular diseases.
DOI of the first publication: 10.3892/mmr.2019.10831
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-384987
ISSN: 1791-3004
Date of registration: 9-Dec-2022
Faculty: M - Medizinische Fakultät
Department: M - Innere Medizin
Professorship: M - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
There are no files associated with this item.

Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.