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Titel: Differential degradation of RNA species by autophagy-related pathways in Arabidopsis
VerfasserIn: Hickl, Daniel
Drews, Franziska
Girke, Christopher
Zimmer, David
Mühlhaus, Timo
Hauth, Jan
Nordström, Karl
Trentmann, Oliver
Neuhaus, Ekkehard H.
Scheuring, David
Fehlmann, Tobias
Keller, Andreas
Simon, Martin
Möhlmann, Torsten
Sprache: Englisch
Titel: Journal of Experimental Botany
Bandnummer: 72
Heft: 20
Seiten: 6867–6881
Verlag/Plattform: Oxford University Press
Erscheinungsjahr: 2021
Freie Schlagwörter: Autophagy
chloroplast
nucleotide
ribosome
RNA
vacuole
DDC-Sachgruppe: 500 Naturwissenschaften
610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: The plant vacuole recycles proteins and RNA delivered to it by autophagy. In this study, by isolating intact vacuoles from Arabidopsis plants, followed by subsequent RNA purification, and deep sequencing, we provide a comprehensive characterization of Arabidopsis vacuolar RNAome. In the vacuolar RNAome, we detected ribosomal RNAs, transfer RNAs, including those of chloroplast origin, and in addition small RNA types. As autophagy is a main mechanism for the transport of RNA to the vacuole, atg5-1 mutants deficient in autophagy were included in our analysis. We observed severely reduced amounts of most chloroplast-derived RNA species in these mutants. Comparisons with cellular RNA composition provided an indication of possible up-regulation of alternative RNA breakdown pathways. By contrast, vacuolar RNA processing and composition in plants lacking vacuolar ribonuclease 2, involved in cellular RNA homeostasis, only showed minor alterations, possibly because of the presence of further so far unknown vacuolar RNase species. Among the small RNA types, we detected mature miRNAs in all vacuolar preparations but at much lower frequency in atg5-1, raising the possibility of a biological role for vacuolar miRNAs.
DOI der Erstveröffentlichung: 10.1093/jxb/erab321
URL der Erstveröffentlichung: http://dx.doi.org/10.1093/jxb/erab321
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-384824
hdl:20.500.11880/34706
http://dx.doi.org/10.22028/D291-38482
ISSN: 1460-2431
0022-0957
Datum des Eintrags: 9-Dez-2022
Bezeichnung des in Beziehung stehenden Objekts: Supplementary data
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https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/jxb/72/20/10.1093_jxb_erab321/1/erab321_suppl_supplementary_figures.pdf?Expires=1673597593&Signature=K3QR8RGxIjd1GozCFrfVqDcVwkPYXCkkQ4htltkVZ6cJ73bUR614T8UutFnN5cWDSTWRgqAkmbpx1I6oVn7yo6zZ7t0ZPA-~m5Jn4RJBZ5woGYplEnN3tacaePwASf9gqyq4U4nsB7GiE8mcmOoDShI72dMNabTWRaeEIq9uz8qJqegA9OwAPqDevL0-VUoM01LtSwU9QsXWHTTcQ3ybiHdZFL7IOhzzAzhrXN~lG0BSrE4gruuWbv9qY3Ceofrchrtvbec-0079DKSgB2xMAEi-PJ7EFcOeoBAajmkUCXbCzdtLD0JwqHhO9OMgkHihx5efVTbhuiBwKUVwYu9DGg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
Fakultät: M - Medizinische Fakultät
NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: M - Medizinische Biometrie, Epidemiologie und medizinische Informatik
NT - Biowissenschaften
Professur: M - Univ.-Prof. Dr. Andreas Keller
NT - Keiner Professur zugeordnet
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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