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Titel: The development of alginate-based amperometric nanoreactors for biochemical profiling of living yeast cells
VerfasserIn: Zolotukhina, E. V.
Katsen-Globa, A.
Koch, Marcus
Fink-Straube, Claudia
Sukmann, T.
Levchenko, M. G.
Silina, Yuliya E.
Sprache: Englisch
Titel: Bioelectrochemistry : official journal of the Bioelectrochemical Society
Bandnummer: 145
Verlag/Plattform: Elsevier
Erscheinungsjahr: 2022
Freie Schlagwörter: Yeast cells
Secondary metabolites
Pd-NPs
Multi-step amperometric detection
Alginate
Nanoreactor
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: This study describes the development of a one-pot electrochemical miniaturized system for simultaneous cultivation and monitoring of the oxidative status of living cells. This system consisted of screen-printed electrodes modified by electroplated Pd-NPs as an electrocatalyst (i) and living yeast cells (Saccharomyces cerevisiae) (ii) immobilized on the cytocompatible alginate layer (iii). Briefly, during the course of electrochemical investigations a novel electroactive compound methylhydrazine derivative as a secondary metabolite and result of microbial activity was found in yeast cells and used as a signaling molecule for their biochemical profiling. Under the optimized experimental conditions the signal corresponding to the found electroactive secondary metabolite formed in medium of living cells was measured without sample collecting, transport, storage or pre-treatment steps (i.e. extraction, pre-concentration, chemical derivatization or labeling). The electrochemical dependencies, which were derived by a miniaturized electroanalytical system, were fully validated in a conventional three-electrode system under inert atmosphere (Ar) and in the presence of oxygen (air, O2). It is believed that the proposed one-pot nanoreactors serving simultaneously as nanofermenters and amperometric detectors for the quantification of secondary metabolites formed in medium of living cells can significantly enhance the understanding of ongoing fermentation processes in the future and our knowledge on the biochemistry of yeasts.
DOI der Erstveröffentlichung: 10.1016/j.bioelechem.2022.108082
URL der Erstveröffentlichung: https://www.sciencedirect.com/science/article/abs/pii/S1567539422000330
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-358277
hdl:20.500.11880/34248
http://dx.doi.org/10.22028/D291-35827
ISSN: 1878-562X
0302-4598
Datum des Eintrags: 8-Nov-2022
Fakultät: NT - Naturwissenschaftlich- Technische Fakultät
Fachrichtung: NT - Biowissenschaften
Professur: NT - Keiner Professur zugeordnet
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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