Please use this identifier to cite or link to this item: doi:10.22028/D291-37798
Title: Urinary extracellular vesicles : A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
Author(s): Erdbrügger, Uta
Blijdorp, Charles J.
Bijnsdorp, Irene V.
Borràs, Francesc E.
Burger, Dylan
Bussolati, Benedetta
Byrd, James Brian
Clayton, Aled
Dear, James W.
Falcón-Pérez, Juan M.
Grange, Cristina
Hill, Andrew F.
Holthöfer, Harry
Hoorn, Ewout J.
Jenster, Guido
Jimenez, Connie R.
Junker, Kerstin
Klein, John
Knepper, Mark A.
Koritzinsky, Erik H.
Luther, James M.
Lenassi, Metka
Leivo, Janne
Mertens, Inge
Musante, Luca
Oeyen, Eline
Puhka, Maija
van Royen, Martin E.
Sánchez, Catherine
Soekmadji, Carolina
Thongboonkerd, Visith
van Steijn, Volkert
Verhaegh, Gerald
Webber, Jason P.
Witwer, Kenneth
Yuen, Peter S.T.
Zheng, Lei
Llorente, Alicia
Martens-Uzunova, Elena S.
Language: English
Title: Journal of Extracellular Vesicles
Volume: 10
Issue: 7
Publisher/Platform: Wiley
Year of Publication: 2021
Free key words: biobank
biomarkers
bladder
extracellular vesicles
kidney
liquid biopsy
prostate
rigor and standardization
urine
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
DOI of the first publication: 10.1002/jev2.12093
URL of the first publication: https://onlinelibrary.wiley.com/doi/10.1002/jev2.12093
Link to this record: urn:nbn:de:bsz:291--ds-377987
hdl:20.500.11880/34184
http://dx.doi.org/10.22028/D291-37798
ISSN: 2001-3078
Date of registration: 3-Nov-2022
Faculty: M - Medizinische Fakultät
Department: M - Urologie und Kinderurologie
Professorship: M - Prof. Dr. Michael Stöckle
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes



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