Please use this identifier to cite or link to this item:
doi:10.22028/D291-37034
Title: | Transcriptional and Epigenetic Consequences of DMSO Treatment on HepaRG Cells |
Author(s): | Dubois-Pot-Schneider, Hélène Aninat, Caroline Kattler, Kathrin Fekir, Karim Jarnouen, Kathleen Cerec, Virginie Glaise, Denise Salhab, Abdulrahman Gasparoni, Gilles Takashi, Kubo Ishida, Seiichi Walter, Jörn Corlu, Anne Walter, Jörn Erik |
Language: | English |
Title: | Cells |
Volume: | 11 |
Issue: | 15 |
Publisher/Platform: | MDPI |
Year of Publication: | 2022 |
Free key words: | DMSO HepaRG cells hepatic differentiation gene expression histone modification |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | Dimethyl sulfoxide (DMSO) is used to sustain or favor hepatocyte differentiation in vitro. Thus, DMSO is used in the differentiation protocol of the HepaRG cells that present the closest drug-metabolizing enzyme activities to primary human hepatocytes in culture. The aim of our study is to clarify its influence on liver-specific gene expression. For that purpose, we performed a large-scale analysis (gene expression and histone modification) to determine the global role of DMSO exposure during the differentiation process of the HepaRG cells. The addition of DMSO drives the upregulation of genes mainly regulated by PXR and PPARα whereas genes not affected by this addition are regulated by HNF1α, HNF4α, and PPARα. DMSO-differentiated-HepaRG cells show a differential expression for genes regulated by histone acetylation, while differentiated-HepaRG cells without DMSO show gene signatures associated with histone deacetylases. In addition, we observed an interplay between cytoskeleton organization and EMC remodeling with hepatocyte maturation. |
DOI of the first publication: | 10.3390/cells11152298 |
Link to this record: | urn:nbn:de:bsz:291--ds-370349 hdl:20.500.11880/33624 http://dx.doi.org/10.22028/D291-37034 |
ISSN: | 2073-4409 |
Date of registration: | 16-Aug-2022 |
Description of the related object: | Supplementary Materials |
Related object: | https://www.mdpi.com/2073-4409/11/15/2298/s1?version=1658840537 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Biowissenschaften |
Professorship: | NT - Prof. Dr. Jörn Walter |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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cells-11-02298.pdf | 4,06 MB | Adobe PDF | View/Open |
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