Please use this identifier to cite or link to this item:
doi:10.22028/D291-36743
Title: | Towards hybrid one-pot/one-electrode Pd-NPs-based nanoreactors for modular biocatalysis |
Author(s): | Koch, M. Apushkinskaya, N. Zolotukhina, E.V. Silina, Y. E. |
Language: | English |
Title: | Biochemical Engineering Journal |
Volume: | 175 |
Publisher/Platform: | Elsevier |
Year of Publication: | 2021 |
Free key words: | One-pot/one-electrode nanobiosensor Multiplexed analysis Small molecular weight bioanalytes Read-out mode |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | Here, fundamental aspects affecting template-assisted engineering of oxidase-associated peroxide oxidation co catalysis of the modeled microanalytical system based on the hybrid palladium nanoparticles (Pd-NPs) with tailored functional properties were studied. By an accurate tuning and validation of the experimental setup, a modular Pd-NPs-doped one-pot/one-electrode amperometric nanobiosensor for advanced multiplex analyte detection was constructed. The specific operational conditions (electrochemical read-out mode, pH, regeneration procedure) of the modular one-pot/one-electrode nanobiosensor allowed a reliable sensing of L-lactate (with linear dynamic range, LDR = 500 µM – 2 mM, R2 = 0.977), D-glucose (with LDR = 200 µM – 50 mM, R2 = 0.987), hydrogen peroxide (with LDR = 20 µM – 100 mM, R2 = 0.998) and glutaraldehyde (with LDR = 1 – 100 mM, R2 = 0.971). In addition, mechanistic aspects influencing the performance of Pd-NPs-doped one-pot/ one-electrode for multiplex analyte sensing were studied in detail. The designed one-pot/one-electrode amper ometric nanobiosensor showed a thin layer electrochemical behavior that greatly enhanced electron transfer between the functional hybrid layer and the electrode. Finally, a specific regeneration procedure of the hybrid one-pot/one-electrode and algorithm towards its usage for modular biocatalysis were developed. The reported strategy can readily be considered as a guideline towards the fabrication of commercialized nanobiosensors with tailored properties for advanced modular biocatalysis. |
DOI of the first publication: | 10.1016/j.bej.2021.108132 |
URL of the first publication: | https://www.sciencedirect.com/science/article/pii/S1369703X21002084 |
Link to this record: | urn:nbn:de:bsz:291--ds-367430 hdl:20.500.11880/33385 http://dx.doi.org/10.22028/D291-36743 |
ISSN: | 1369-703X |
Date of registration: | 11-Jul-2022 |
Description of the related object: | Supplementary material |
Related object: | https://ars.els-cdn.com/content/image/1-s2.0-S1369703X21002084-mmc1.docx |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Biowissenschaften |
Professorship: | NT - Prof. Dr. Bruce Morgan |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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File | Description | Size | Format | |
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1-s2.0-S1369703X21002084-main.pdf | 6,27 MB | Adobe PDF | View/Open |
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