Please use this identifier to cite or link to this item: doi:10.22028/D291-36743
Title: Towards hybrid one-pot/one-electrode Pd-NPs-based nanoreactors for modular biocatalysis
Author(s): Koch, M.
Apushkinskaya, N.
Zolotukhina, E.V.
Silina, Y. E.
Language: English
Title: Biochemical Engineering Journal
Volume: 175
Publisher/Platform: Elsevier
Year of Publication: 2021
Free key words: One-pot/one-electrode nanobiosensor
Multiplexed analysis
Small molecular weight bioanalytes
Read-out mode
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Here, fundamental aspects affecting template-assisted engineering of oxidase-associated peroxide oxidation co catalysis of the modeled microanalytical system based on the hybrid palladium nanoparticles (Pd-NPs) with tailored functional properties were studied. By an accurate tuning and validation of the experimental setup, a modular Pd-NPs-doped one-pot/one-electrode amperometric nanobiosensor for advanced multiplex analyte detection was constructed. The specific operational conditions (electrochemical read-out mode, pH, regeneration procedure) of the modular one-pot/one-electrode nanobiosensor allowed a reliable sensing of L-lactate (with linear dynamic range, LDR = 500 µM – 2 mM, R2 = 0.977), D-glucose (with LDR = 200 µM – 50 mM, R2 = 0.987), hydrogen peroxide (with LDR = 20 µM – 100 mM, R2 = 0.998) and glutaraldehyde (with LDR = 1 – 100 mM, R2 = 0.971). In addition, mechanistic aspects influencing the performance of Pd-NPs-doped one-pot/ one-electrode for multiplex analyte sensing were studied in detail. The designed one-pot/one-electrode amper ometric nanobiosensor showed a thin layer electrochemical behavior that greatly enhanced electron transfer between the functional hybrid layer and the electrode. Finally, a specific regeneration procedure of the hybrid one-pot/one-electrode and algorithm towards its usage for modular biocatalysis were developed. The reported strategy can readily be considered as a guideline towards the fabrication of commercialized nanobiosensors with tailored properties for advanced modular biocatalysis.
DOI of the first publication: 10.1016/j.bej.2021.108132
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-367430
ISSN: 1369-703X
Date of registration: 11-Jul-2022
Description of the related object: Supplementary material
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Bruce Morgan
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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