Please use this identifier to cite or link to this item: doi:10.22028/D291-36703
Title: A murine model to study vasoreactivity and intravascular flow in lung isograft microvessels
Author(s): Regelin, Nora
Heyder, Susanne
Laschke, Matthias W.
Hadizamani, Yalda
Borgmann, Michèle
Moehrlen, Ueli
Schramm, René
Bals, Robert
Menger, Michael D.
Hamacher, Jürg
Language: English
Title: Scientific Reports
Volume: 9
Issue: 1
Publisher/Platform: Springer Nature
Year of Publication: 2019
Free key words: Epithelial–mesenchymal transition
Experimental models of disease
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Intravital microscopy of orthotopic lung tissue is technically demanding, especially for repeated investigations. Therefore, we have established a novel approach, which allows non-invasive repetitive in vivo microscopy of ectopic lung tissue in dorsal skinfold chambers. Syngeneic subpleural peripheral lung tissue and autologous endometrium (control) were transplanted onto the striated muscle within dorsal skinfold chambers of C57BL/6 mice. Grafts were analysed by intravital fuorescence microscopy over 14 days. Angiogenesis occurred in the grafts on day 3, as indicated by sinusoidal microvessels on the grafts’ edges with very slow blood fow, perifocal oedema, and haemorrhage. By day 10, lung transplants were completely revascularized, exhibited a dense network of microvessels with irregular diameters, chaotic angioarchitecture, and high blood fow. Compared to lung tissue, endometrial grafts contained a structured, glomerulus-like vessel architecture with lower blood fow. Despite missing ventilation, hypoxic vasoconstriction of the lung tissue arterioles occurred. In contrast, endometrium tissue arterioles dilated during hypoxia and constricted in hyperoxia. This demonstrates that ectopic lung grafts keep their ability for organ-specifc hypoxic vasoconstriction. These fndings indicate that our approach is suitable for repetitive in vivo pulmonary microcirculation analyses. The high blood fow and hypoxia-induced vasoconstriction in lung grafts suggest a physiological intrinsic vasoregulation independent of the recipient tissue.
DOI of the first publication: 10.1038/s41598-019-41590-7
URL of the first publication: https://www.nature.com/articles/s41598-019-41590-7
Link to this record: urn:nbn:de:bsz:291--ds-367032
hdl:20.500.11880/33347
http://dx.doi.org/10.22028/D291-36703
ISSN: 2045-2322
Date of registration: 8-Jul-2022
Faculty: M - Medizinische Fakultät
Department: M - Chirurgie
M - Innere Medizin
Professorship: M - Prof. Dr. Robert Bals
M - Prof. Dr. Michael D. Menger
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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