Please use this identifier to cite or link to this item:
doi:10.22028/D291-36703
Title: | A murine model to study vasoreactivity and intravascular flow in lung isograft microvessels |
Author(s): | Regelin, Nora Heyder, Susanne Laschke, Matthias W. Hadizamani, Yalda Borgmann, Michèle Moehrlen, Ueli Schramm, René Bals, Robert Menger, Michael D. Hamacher, Jürg |
Language: | English |
Title: | Scientific Reports |
Volume: | 9 |
Issue: | 1 |
Publisher/Platform: | Springer Nature |
Year of Publication: | 2019 |
Free key words: | Epithelial–mesenchymal transition Experimental models of disease |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | Intravital microscopy of orthotopic lung tissue is technically demanding, especially for repeated investigations. Therefore, we have established a novel approach, which allows non-invasive repetitive in vivo microscopy of ectopic lung tissue in dorsal skinfold chambers. Syngeneic subpleural peripheral lung tissue and autologous endometrium (control) were transplanted onto the striated muscle within dorsal skinfold chambers of C57BL/6 mice. Grafts were analysed by intravital fuorescence microscopy over 14 days. Angiogenesis occurred in the grafts on day 3, as indicated by sinusoidal microvessels on the grafts’ edges with very slow blood fow, perifocal oedema, and haemorrhage. By day 10, lung transplants were completely revascularized, exhibited a dense network of microvessels with irregular diameters, chaotic angioarchitecture, and high blood fow. Compared to lung tissue, endometrial grafts contained a structured, glomerulus-like vessel architecture with lower blood fow. Despite missing ventilation, hypoxic vasoconstriction of the lung tissue arterioles occurred. In contrast, endometrium tissue arterioles dilated during hypoxia and constricted in hyperoxia. This demonstrates that ectopic lung grafts keep their ability for organ-specifc hypoxic vasoconstriction. These fndings indicate that our approach is suitable for repetitive in vivo pulmonary microcirculation analyses. The high blood fow and hypoxia-induced vasoconstriction in lung grafts suggest a physiological intrinsic vasoregulation independent of the recipient tissue. |
DOI of the first publication: | 10.1038/s41598-019-41590-7 |
URL of the first publication: | https://www.nature.com/articles/s41598-019-41590-7 |
Link to this record: | urn:nbn:de:bsz:291--ds-367032 hdl:20.500.11880/33347 http://dx.doi.org/10.22028/D291-36703 |
ISSN: | 2045-2322 |
Date of registration: | 8-Jul-2022 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Chirurgie M - Innere Medizin |
Professorship: | M - Prof. Dr. Robert Bals M - Prof. Dr. Michael D. Menger |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
---|---|---|---|---|
s41598-019-41590-7.pdf | 1,87 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License