Please use this identifier to cite or link to this item:
doi:10.22028/D291-36573
Title: | Molecular Characterization of Cancer Associated Fibroblasts in Prostate Cancer |
Author(s): | Vitale, Giovanni Caraglia, Michele Jung, Volker Kamradt, Jörn Gentilini, Davide Di Martino, Maria Teresa Dicitore, Alessandra Abate, Marianna Tagliaferri, Pierosandro Itro, Annalisa Ferro, Matteo Balsamo, Raffaele De Sio, Marco Facchini, Gaetano Persani, Luca Schmitt, Kai Saar, Matthias Stöckle, Michael Unteregger, Gerhard Zappavigna, Silvia |
Language: | English |
Title: | Cancers |
Volume: | 14 |
Issue: | 12 |
Publisher/Platform: | MDPI |
Year of Publication: | 2022 |
Free key words: | androgen signaling cancer-associated fibroblasts cell proliferation non cancer-associated fibroblasts prostate cancer transcriptomic profiling |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | Background: Stromal components surrounding epithelial cancer cells seem to play a pivotal role during epithelial-to-mesenchymal transition (EMT), tumor invasion, and metastases. To identify the molecular mechanisms underlying tumor–stroma interactions may yield novel therapeutic targets for prostate cancer. Methods: Gene expression profile of prostate-cancer associated fibroblast (PCAF) and prostate non-cancer associated fibroblast (PNAF) cells isolated from radical prostatectomy was performed by Illumina, analyzed, and further processed by Ingenuity®: IPA® software. qRT-PCR was performed on an independent set of 17 PCAF, 12 PNAF, and 12 fibroblast cell lines derived from patients with benign prostatic hyperplasia (BPHF). Results: Using microarray analysis, we found six upregulated genes and two downregulated genes in PCAFs compared to PNAFs. To validate microarray results, we performed qRT-PCR for the most significantly regulated genes involved in the modulation of proliferation and androgen resistance on an independent set of PNAF, PCAF, and BHPF samples. We confirmed the increased expression of SCARB1, MAPK3K1, and TGF-β as well as the decreased expression of S100A10 in PCAFs compared to PNAFs and BPHFs. Conclusions: These results provide strong evidence that the observed changes in the gene expression profile of PCAFs can contribute to functional alteration of adjacent prostate cancer cells. |
DOI of the first publication: | 10.3390/cancers14122943 |
Link to this record: | urn:nbn:de:bsz:291--ds-365732 hdl:20.500.11880/33225 http://dx.doi.org/10.22028/D291-36573 |
ISSN: | 2072-6694 |
Date of registration: | 24-Jun-2022 |
Description of the related object: | Supplementary Materials |
Related object: | https://www.mdpi.com/article/10.3390/cancers14122943/s1 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Pathologie M - Urologie und Kinderurologie |
Professorship: | M - Prof. Dr. Michael Stöckle M - Keiner Professur zugeordnet |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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cancers-14-02943.pdf | 2,46 MB | Adobe PDF | View/Open |
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