Please use this identifier to cite or link to this item:
doi:10.22028/D291-35971
Title: | Effectiveness of Direct Laser Interference Patterning and Peptide Immobilization on Endothelial Cell Migration for Cardio-Vascular Applications: An In Vitro Study |
Author(s): | Schieber, Romain Mas-Moruno, Carlos Lasserre, Federico Roa, Joan Josep Ginebra, Maria-Pau Mücklich, Frank Pegueroles, Marta |
Language: | English |
Title: | Nanomaterials |
Volume: | 12 |
Issue: | 7 |
Publisher/Platform: | MDPI |
Year of Publication: | 2022 |
Free key words: | direct laser interference patterning (DLIP) cobalt-chromium alloy biofunctionalization cell adhesive peptides endothelial cell migration |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | Endothelial coverage of an exposed cardiovascular stent surface leads to the occurrence of restenosis and late-stent thrombosis several months after implantation. To overcome this difficulty, modification of stent surfaces with topographical or biochemical features may be performed to increase endothelial cells’ (ECs) adhesion and/or migration. This work combines both strategies on cobalt-chromium (CoCr) alloy and studies the potential synergistic effect of linear patterned surfaces that are obtained by direct laser interference patterning (DLIP), coupled with the use of Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) peptides. An extensive characterization of the modified surfaces was performed by using AFM, XPS, surface charge, electrochemical analysis and fluorescent methods. The biological response was studied in terms of EC adhesion, migration and proliferation assays. CoCr surfaces were successfully patterned with a periodicity of 10 µm and two different depths, D (≈79 and 762 nm). RGD and YIGSR were immobilized on the surfaces by CPTES silanization. Early EC adhesion was increased on the peptide-functionalized surfaces, especially for YIGSR compared to RGD. High-depth patterns generated 80% of ECs’ alignment within the topographical lines and enhanced EC migration. It is noteworthy that the combined use of the two strategies synergistically accelerated the ECs’ migration and proliferation, proving the potential of this strategy to enhance stent endothelialization. |
DOI of the first publication: | 10.3390/nano12071217 |
Link to this record: | urn:nbn:de:bsz:291--ds-359715 hdl:20.500.11880/32787 http://dx.doi.org/10.22028/D291-35971 |
ISSN: | 2079-4991 |
Date of registration: | 12-Apr-2022 |
Description of the related object: | Supplementary Materials |
Related object: | https://www.mdpi.com/article/10.3390/nano12071217/s1 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Materialwissenschaft und Werkstofftechnik |
Professorship: | NT - Prof. Dr. Frank Mücklich |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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nanomaterials-12-01217.pdf | 3,51 MB | Adobe PDF | View/Open |
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