Please use this identifier to cite or link to this item: doi:10.22028/D291-35971
Title: Effectiveness of Direct Laser Interference Patterning and Peptide Immobilization on Endothelial Cell Migration for Cardio-Vascular Applications: An In Vitro Study
Author(s): Schieber, Romain
Mas-Moruno, Carlos
Lasserre, Federico
Roa, Joan Josep
Ginebra, Maria-Pau
Mücklich, Frank
Pegueroles, Marta
Language: English
Title: Nanomaterials
Volume: 12
Issue: 7
Publisher/Platform: MDPI
Year of Publication: 2022
Free key words: direct laser interference patterning (DLIP)
cobalt-chromium alloy
cell adhesive peptides
endothelial cell migration
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Endothelial coverage of an exposed cardiovascular stent surface leads to the occurrence of restenosis and late-stent thrombosis several months after implantation. To overcome this difficulty, modification of stent surfaces with topographical or biochemical features may be performed to increase endothelial cells’ (ECs) adhesion and/or migration. This work combines both strategies on cobalt-chromium (CoCr) alloy and studies the potential synergistic effect of linear patterned surfaces that are obtained by direct laser interference patterning (DLIP), coupled with the use of Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) peptides. An extensive characterization of the modified surfaces was performed by using AFM, XPS, surface charge, electrochemical analysis and fluorescent methods. The biological response was studied in terms of EC adhesion, migration and proliferation assays. CoCr surfaces were successfully patterned with a periodicity of 10 µm and two different depths, D (≈79 and 762 nm). RGD and YIGSR were immobilized on the surfaces by CPTES silanization. Early EC adhesion was increased on the peptide-functionalized surfaces, especially for YIGSR compared to RGD. High-depth patterns generated 80% of ECs’ alignment within the topographical lines and enhanced EC migration. It is noteworthy that the combined use of the two strategies synergistically accelerated the ECs’ migration and proliferation, proving the potential of this strategy to enhance stent endothelialization.
DOI of the first publication: 10.3390/nano12071217
Link to this record: urn:nbn:de:bsz:291--ds-359715
ISSN: 2079-4991
Date of registration: 12-Apr-2022
Description of the related object: Supplementary Materials
Related object:
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Materialwissenschaft und Werkstofftechnik
Professorship: NT - Prof. Dr. Frank Mücklich
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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