Please use this identifier to cite or link to this item: doi:10.22028/D291-35411
Title: A comprehensive profile of circulating RNAs in human serum
Author(s): Umu, Sinan Uğur
Langseth, Hilde
Bucher-Johannessen, Cecilie
Fromm, Bastian
Keller, Andreas
Meese, Eckart
Lauritzen, Marianne
Leithaug, Magnus
Lyle, Robert
Rounge, Trine B.
Language: English
Title: RNA Biology
Volume: 15
Issue: 2
Pages: 242–250
Publisher/Platform: Taylor & Francis
Year of Publication: 2017
Free key words: Small RNA
rna sequencing
circulating RNA
RNA fragments
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Non-coding RNA (ncRNA) molecules have fundamental roles in cells and many are also stable in body fluids as extracellular RNAs. In this study, we used RNA sequencing (RNA-seq) to investigate the profile of small non-coding RNA (sncRNA) in human serum. We analyzed 10 billion Illumina reads from 477 serum samples, included in the Norwegian population-based Janus Serum Bank (JSB). We found that the core serum RNA repertoire includes 258 micro RNAs (miRNA), 441 piwi-interacting RNAs (piRNA), 411 transfer RNAs (tRNA), 24 small nucleolar RNAs (snoRNA), 125 small nuclear RNAs (snRNA) and 123 miscellaneous RNAs (misc-RNA). We also investigated biological and technical variation in expression, and the results suggest that many RNA molecules identified in serum contain signs of biological variation. They are therefore unlikely to be random degradation by-products. In addition, the presence of specific fragments of tRNA, snoRNA, Vault RNA and Y_RNA indicates protection from degradation. Our results suggest that many circulating RNAs in serum can be potential biomarkers.
DOI of the first publication: 10.1080/15476286.2017.1403003
Link to this record: urn:nbn:de:bsz:291--ds-354115
ISSN: 1555-8584
Date of registration: 4-Feb-2022
Description of the related object: Supplemental material
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Humangenetik
M - Medizinische Biometrie, Epidemiologie und medizinische Informatik
Professorship: M - Univ.-Prof. Dr. Andreas Keller
M - Prof. Dr. Eckhart Meese
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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