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doi:10.22028/D291-34513
Title: | High Glucose Enhances Cytotoxic T Lymphocyte-Mediated Cytotoxicity |
Author(s): | Zhu, Jie Yang, Wenjuan Zhou, Xiangda Zöphel, Dorina Soriano-Baguet, Leticia Dolgener, Denise Carlein, Christopher Hof, Chantal Zhao, Renping Ye, Shandong Schwarz, Eva C Brenner, Dirk Prates Roma, Leticia Qu, Bin |
Language: | English |
Title: | Frontiers in immunology |
Volume: | 12 |
Publisher/Platform: | Frontiers |
Year of Publication: | 2021 |
Publikation type: | Journal Article |
Abstract: | Cytotoxic T lymphocytes (CTLs) are key players to eliminate tumorigenic or pathogen-infected cells using lytic granules (LG) and Fas ligand (FasL) pathways. Depletion of glucose leads to severely impaired cytotoxic function of CTLs. However, the impact of excessive glucose on CTL functions still remains largely unknown. Here we used primary human CD8+ T cells, which were stimulated by CD3/CD28 beads and cultured in medium either containing high glucose (HG, 25 mM) or normal glucose (NG, 5.6 mM). We found that in HG-CTLs, glucose uptake and glycolysis were enhanced, whereas proliferation remained unaltered. Furthermore, CTLs cultured in HG exhibited an enhanced CTL killing efficiency compared to their counterparts in NG. Unexpectedly, expression of cytotoxic proteins (perforin, granzyme A, granzyme B and FasL), LG release, cytokine/cytotoxic protein release and CTL migration remained unchanged in HG-cultured CTLs. Interestingly, additional extracellular Ca2+ diminished HG-enhanced CTL killing function. Our findings suggest that in an environment with excessive glucose, CTLs could eliminate target cells more efficiently, at least for a certain period of time, in a Ca2+-dependent manner. |
DOI of the first publication: | 10.3389/fimmu.2021.689337 |
URL of the first publication: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.689337/full |
Link to this record: | hdl:20.500.11880/31697 http://dx.doi.org/10.22028/D291-34513 |
ISSN: | 1664-3224 |
Date of registration: | 3-Sep-2021 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Biophysik |
Professorship: | M - Keiner Professur zugeordnet |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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