Please use this identifier to cite or link to this item:
doi:10.22028/D291-34582
Title: | EPR Study of KO2 as a Source of Superoxide and •BMPO-OH/OOH Radical That Cleaves Plasmid DNA and Detects Radical Interaction with H2S and Se-Derivatives |
Author(s): | Misak, Anton Brezova, Vlasta Chovanec, Miroslav Luspai, Karol Nasim, Muhammad Jawad Grman, Marian Tomasova, Lenka Jacob, Claus Ondrias, Karol |
Language: | English |
Title: | Antioxidants |
Volume: | 10 |
Issue: | 8 |
Publisher/Platform: | MDPI |
Year of Publication: | 2021 |
Free key words: | KO2 antioxidants EPR spectra simulation BMPO-OOH spin adduct superoxide radical hydrogen sulfide selenium-derivatives cleavage DNA |
DDC notations: | 500 Science |
Publikation type: | Journal Article |
Abstract: | : Superoxide radical anion (O2 •−) and its derivatives regulate numerous physiological and pathological processes, which are extensively studied. The aim of our work was to utilize KO2 as a source of O2 •− and the electron paramagnetic resonance (EPR) spin trapping 5-tert-butoxycarbonyl-5- methyl-1-pyrroline N-oxide (BMPO) technique for the preparation of •BMPO-OOH and/or •BMPOOH radicals in water solution without DMSO. The method distinguishes the interactions of various compounds with •BMPO-OOH and/or •BMPO-OH radicals over time. Here, we show that the addition of a buffered BMPO-HCl mixture to powdered KO2 formed relatively stable •BMPO-OOH and •BMPO-OH radicals and H2O2 , where the •BMPO-OOH/OH ratio depended on the pH. At a final pH of ~6.5–8.0, the concentration of •BMPO-OOH radicals was ≥20 times higher than that of •BMPO-OH, whereas at pH 9.0–10.0, the •BMPO-OH radicals prevailed. The •BMPO-OOH/OH radicals effectively cleaved the plasmid DNA. H2S decreased the concentration of •BMPO-OOH/OH radicals, whereas the selenium derivatives 1-methyl-4-(3-(phenylselanyl) propyl) piperazine and 1-methyl-4-(4-(phenylselanyl) butyl) piperazine increased the proportion of •BMPO-OH over the •BMPO-OOH radicals. In conclusion, the presented approach of using KO2 as a source of O2 •−/H2O2 and EPR spin trap BMPO for the preparation of •BMPO-OOH/OH radicals in a physiological solution could be useful to study the biological effects of radicals and their interactions with compounds. |
DOI of the first publication: | 10.3390/antiox10081286 |
Link to this record: | urn:nbn:de:bsz:291--ds-345829 hdl:20.500.11880/31655 http://dx.doi.org/10.22028/D291-34582 |
ISSN: | 2076-3921 |
Date of registration: | 27-Aug-2021 |
Description of the related object: | Supplementary Materials |
Related object: | https://www.mdpi.com/article/10.3390/antiox10081286/s1 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Pharmazie |
Professorship: | NT - Prof. Dr. Claus Jacob |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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antioxidants-10-01286.pdf | 6,69 MB | Adobe PDF | View/Open |
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