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doi:10.22028/D291-34219
Titel: | Mitochondrial nicotinamide adenine dinucleotide hydride dehydrogenase (NADH) subunit 4 (MTND4) polymorphisms and their association with male infertility |
VerfasserIn: | Dahadhah, Fatina W. Jaweesh, Mayyas Saleh Al Zoubi, Mazhar Salim Alarjah, Manal Issam Abu Hammadeh, Mohamad Eid Amor, Houda |
Sprache: | Englisch |
Titel: | Journal of Assisted Reproduction and Genetics |
Verlag/Plattform: | Springer Nature |
Erscheinungsjahr: | 2021 |
Freie Schlagwörter: | mtDNA MTND4 SNP Male infertility |
DDC-Sachgruppe: | 610 Medizin, Gesundheit |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | Purpose The purpose of the present study was to determine the relationship between infertility and the polymorphisms of mitochondrial NADH dehydrogenase subunit 4 (MTND4) by spermatozoa analysis in fertile and subfertile men. Methods Samples were divided into 68 subfertile men (case group) and 44 fertile men (control group). After semen analysis, samples were purified. The whole genome was extracted using a QIAamp DNA Mini Kit and the mitochondrial DNA was amplified by using the REPLI-g Mitochondrial DNA Kit. Polymerase chain reaction (PCR) was used to amplify the MT-ND4 gene. Then, samples were purified and sequenced using the Sanger method. Results Twenty-five single-nucleotide polymorphisms (SNPs) were identified in the MTND4 gene. The genotype frequencies of the study population showed a statistically significant association between rs2853495 G>A (Gly320Gly) and male infertility (P = 0.0351). Similarly, the allele frequency test showed that rs2853495 G>A (Gly320Gly) and rs869096886 A>G (Leu164Leu) were significantly associated with male infertility (adjusted OR = 2.616, 95% CI = 1.374–4.983, P = 0.002; adjusted OR = 2.237, 95% CI = 1.245–4.017, P = 0.007, respectively). Conclusion In conclusion, our findings suggested that male infertility was correlated with rs2853495 and rs869096886 SNPs in MTND4. |
DOI der Erstveröffentlichung: | 10.1007/s10815-021-02199-w |
Link zu diesem Datensatz: | urn:nbn:de:bsz:291--ds-342199 hdl:20.500.11880/31402 http://dx.doi.org/10.22028/D291-34219 |
ISSN: | 1573-7330 1058-0468 |
Datum des Eintrags: | 22-Jun-2021 |
Bezeichnung des in Beziehung stehenden Objekts: | Supplementary Information |
In Beziehung stehendes Objekt: | https://static-content.springer.com/esm/art%3A10.1007%2Fs10815-021-02199-w/MediaObjects/10815_2021_2199_MOESM1_ESM.doc |
Fakultät: | M - Medizinische Fakultät |
Fachrichtung: | M - Frauenheilkunde |
Professur: | M - Prof. Dr. E.-F. Solomayer |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Datei | Beschreibung | Größe | Format | |
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Dahadhah2021_Article_MitochondrialNicotinamideAdeni.pdf | 337,79 kB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons