Please use this identifier to cite or link to this item: doi:10.22028/D291-34219
Title: Mitochondrial nicotinamide adenine dinucleotide hydride dehydrogenase (NADH) subunit 4 (MTND4) polymorphisms and their association with male infertility
Author(s): Dahadhah, Fatina W.
Jaweesh, Mayyas Saleh
Al Zoubi, Mazhar Salim
Alarjah, Manal Issam Abu
Hammadeh, Mohamad Eid
Amor, Houda
Language: English
Title: Journal of Assisted Reproduction and Genetics
Publisher/Platform: Springer Nature
Year of Publication: 2021
Free key words: mtDNA
Male infertility
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Purpose The purpose of the present study was to determine the relationship between infertility and the polymorphisms of mitochondrial NADH dehydrogenase subunit 4 (MTND4) by spermatozoa analysis in fertile and subfertile men. Methods Samples were divided into 68 subfertile men (case group) and 44 fertile men (control group). After semen analysis, samples were purified. The whole genome was extracted using a QIAamp DNA Mini Kit and the mitochondrial DNA was amplified by using the REPLI-g Mitochondrial DNA Kit. Polymerase chain reaction (PCR) was used to amplify the MT-ND4 gene. Then, samples were purified and sequenced using the Sanger method. Results Twenty-five single-nucleotide polymorphisms (SNPs) were identified in the MTND4 gene. The genotype frequencies of the study population showed a statistically significant association between rs2853495 G>A (Gly320Gly) and male infertility (P = 0.0351). Similarly, the allele frequency test showed that rs2853495 G>A (Gly320Gly) and rs869096886 A>G (Leu164Leu) were significantly associated with male infertility (adjusted OR = 2.616, 95% CI = 1.374–4.983, P = 0.002; adjusted OR = 2.237, 95% CI = 1.245–4.017, P = 0.007, respectively). Conclusion In conclusion, our findings suggested that male infertility was correlated with rs2853495 and rs869096886 SNPs in MTND4.
DOI of the first publication: 10.1007/s10815-021-02199-w
Link to this record: urn:nbn:de:bsz:291--ds-342199
ISSN: 1573-7330
Date of registration: 22-Jun-2021
Description of the related object: Supplementary Information
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Frauenheilkunde
Professorship: M - Prof. Dr. E.-F. Solomayer
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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