Please use this identifier to cite or link to this item:
doi:10.22028/D291-30568
Title: | Targeted Deletion of PTEN in Kisspeptin Cells Results in Brain Region- and Sex-Specific Effects on Kisspeptin Expression and Gonadotropin Release |
Author(s): | Negrón, Ariel L. Yu, Guiqin Boehm, Ulrich Acosta-Martínez, Maricedes |
Language: | English |
Title: | International Journal of Molecular Sciences |
Volume: | 21 |
Issue: | 6 |
Publisher/Platform: | MDPI |
Year of Publication: | 2020 |
Free key words: | PTEN luteinizing hormone mTOR kisspeptin hypothalamus anteroventral periventricular nucleus arcuate nucleus |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | Kisspeptin-expressing neurons in the anteroventral periventricular nucleus (AVPV) and the arcuate nucleus (ARC) of the hypothalamus relay hormonal and metabolic information to gonadotropin-releasing hormone neurons, which in turn regulate pituitary and gonadal function. Phosphatase and tensin homolog (PTEN) blocks phosphatidylinositol 3-kinase (PI3K), a signaling pathway utilized by peripheral factors to transmit their signals. However, whether PTEN signaling in kisspeptin neurons helps to integrate peripheral hormonal cues to regulate gonadotropin release is unknown. To address this question, we generated mice with a kisspeptin cell-specific deletion of Pten (Kiss-PTEN KO), and first assessed kisspeptin protein expression and gonadotropin release in these animals. Kiss-PTEN KO mice displayed a profound sex and region-specific kisspeptin neuron hyperthrophy. We detected both kisspeptin neuron hyperthrophy as well as increased kisspeptin fiber densities in the AVPV and ARC of Kiss-PTEN KO females and in the ARC of Kiss-PTEN KO males. Moreover, Kiss-PTEN KO mice showed a reduced gonadotropin release in response to gonadectomy. We also found a hyperactivation of mTOR, a downstream PI3K target and central regulator of cell metabolism, in the AVPV and ARC of Kiss-PTEN KO females but not males. Fasting, known to inhibit hypothalamic kisspeptin expression and luteinizing hormone levels, failed to induce these changes in Kiss-PTEN KO females. We conclude that PTEN signaling regulates kisspeptin protein synthesis in both sexes and that its role as a metabolic signaling molecule in kisspeptin neurons is sex-specific. |
DOI of the first publication: | 10.3390/ijms21062107 |
Link to this record: | urn:nbn:de:bsz:291--ds-305681 hdl:20.500.11880/30215 http://dx.doi.org/10.22028/D291-30568 |
ISSN: | 1422-0067 |
Date of registration: | 16-Dec-2020 |
Description of the related object: | Supplementary Materials |
Related object: | http://www.mdpi.com/1422-0067/21/6/2107/s1 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Experimentelle und Klinische Pharmakologie und Toxikologie |
Professorship: | M - Prof. Dr. Ulrich Boehm |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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ijms-21-02107.pdf | 4,85 MB | Adobe PDF | View/Open |
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