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Titel: Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis
VerfasserIn: Lapitz, Ainhoa
Arbelaiz, Ander
O’Rourke, Colm J.
Lavin, Jose L.
Casta, Adelaida La
Ibarra, Cesar
Jimeno, Juan P.
Santos-Laso, Alvaro
Izquierdo-Sanchez, Laura
Krawczyk, Marcin
Perugorria, Maria J.
Jimenez-Aguero, Raul
Sanchez-Campos, Alberto
Riaño, Ioana
Gónzalez, Esperanza
Lammert, Frank
Marzioni, Marco
Macias, Rocio I.R.
Marin, Jose J. G.
Karlsen, Tom H.
Bujanda, Luis
Falcón-Pérez, Juan M.
Andersen, Jesper B.
Aransay, Ana M.
Rodrigues, Pedro M.
Banales, Jesus M.
Sprache: Englisch
Titel: Cells
Bandnummer: 9
Heft: 3
Verlag/Plattform: MDPI
Erscheinungsjahr: 2020
Freie Schlagwörter: biomarkers
cholangiocarcinoma
extracellular vesicles
liquid biopsy
transcriptomics
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies of most CCAs are unknown, but primary sclerosing cholangitis (PSC) is a risk factor. Non-invasive diagnosis of CCA is challenging and accurate biomarkers are lacking. We aimed to characterize the transcriptomic profile of serum and urine extracellular vesicles (EVs) from patients with CCA, PSC, ulcerative colitis (UC), and healthy individuals. Serum and urine EVs were isolated by serial ultracentrifugations and characterized by nanoparticle tracking analysis, transmission electron microscopy, and immunoblotting. EVs transcriptome was determined by Illumina gene expression array [messenger RNAs (mRNA) and non-coding RNAs (ncRNAs)]. Differential RNA profiles were found in serum and urine EVs from patients with CCA compared to control groups (disease and healthy), showing high diagnostic capacity. The comparison of the mRNA profiles of serum or urine EVs from patients with CCA with the transcriptome of tumor tissues from two cohorts of patients, CCA cells in vitro, and CCA cellsderived EVs, identified 105 and 39 commonly-altered transcripts, respectively. Gene ontology analysis indicated that most commonly-altered mRNAs participate in carcinogenic steps. Overall, patients with CCA present specific RNA profiles in EVs mirroring the tumor, and constituting novel promising liquid biopsy biomarkers.
DOI der Erstveröffentlichung: 10.3390/cells9030721
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-305614
hdl:20.500.11880/30208
http://dx.doi.org/10.22028/D291-30561
ISSN: 2073-4409
Datum des Eintrags: 15-Dez-2020
Bezeichnung des in Beziehung stehenden Objekts: Supplementary Materials
In Beziehung stehendes Objekt: https://www.mdpi.com/2073-4409/9/3/721/s1
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Innere Medizin
Professur: M - Prof. Dr. Frank Lammert
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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