Please use this identifier to cite or link to this item: doi:10.22028/D291-30561
Title: Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis
Author(s): Lapitz, Ainhoa
Arbelaiz, Ander
O’Rourke, Colm J.
Lavin, Jose L.
Casta, Adelaida La
Ibarra, Cesar
Jimeno, Juan P.
Santos-Laso, Alvaro
Izquierdo-Sanchez, Laura
Krawczyk, Marcin
Perugorria, Maria J.
Jimenez-Aguero, Raul
Sanchez-Campos, Alberto
Riaño, Ioana
Gónzalez, Esperanza
Lammert, Frank
Marzioni, Marco
Macias, Rocio I.R.
Marin, Jose J. G.
Karlsen, Tom H.
Bujanda, Luis
Falcón-Pérez, Juan M.
Andersen, Jesper B.
Aransay, Ana M.
Rodrigues, Pedro M.
Banales, Jesus M.
Language: English
Title: Cells
Volume: 9
Issue: 3
Publisher/Platform: MDPI
Year of Publication: 2020
Free key words: biomarkers
extracellular vesicles
liquid biopsy
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies of most CCAs are unknown, but primary sclerosing cholangitis (PSC) is a risk factor. Non-invasive diagnosis of CCA is challenging and accurate biomarkers are lacking. We aimed to characterize the transcriptomic profile of serum and urine extracellular vesicles (EVs) from patients with CCA, PSC, ulcerative colitis (UC), and healthy individuals. Serum and urine EVs were isolated by serial ultracentrifugations and characterized by nanoparticle tracking analysis, transmission electron microscopy, and immunoblotting. EVs transcriptome was determined by Illumina gene expression array [messenger RNAs (mRNA) and non-coding RNAs (ncRNAs)]. Differential RNA profiles were found in serum and urine EVs from patients with CCA compared to control groups (disease and healthy), showing high diagnostic capacity. The comparison of the mRNA profiles of serum or urine EVs from patients with CCA with the transcriptome of tumor tissues from two cohorts of patients, CCA cells in vitro, and CCA cellsderived EVs, identified 105 and 39 commonly-altered transcripts, respectively. Gene ontology analysis indicated that most commonly-altered mRNAs participate in carcinogenic steps. Overall, patients with CCA present specific RNA profiles in EVs mirroring the tumor, and constituting novel promising liquid biopsy biomarkers.
DOI of the first publication: 10.3390/cells9030721
Link to this record: urn:nbn:de:bsz:291--ds-305614
ISSN: 2073-4409
Date of registration: 15-Dec-2020
Description of the related object: Supplementary Materials
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Innere Medizin
Professorship: M - Prof. Dr. Frank Lammert
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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