Please use this identifier to cite or link to this item: doi:10.22028/D291-32447
Title: Molecular epidemiology and antimicrobial resistance of Clostridioides difficile detected in chicken, soil and human samples from Zimbabwe
Author(s): Berger, Fabian K.
Mellmann, Alexander
Bischoff, Markus
von Müller, Lutz
Becker, Sören L.
Simango, Clifford
Gärtner, Barbara
Language: English
Title: International Journal of Infectious Diseases
Volume: 96
Startpage: 82
Endpage: 87
Publisher/Platform: Elsevier
Year of Publication: 2020
Free key words: Epidemiology
Southern Africa
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: Background: Clostridioides difficile is the major cause of infectious nosocomial diarrhoea in industrialized nations. Data on the occurrence of C. difficile in Africa, ribotype (RT) distribution, antimicrobial susceptibility patterns and potential zoonotic transmission are scarce. Methods: 80 Zimbabwean C. difficile isolates from different sources (chicken [n = 30], soil [n = 21] and humans [n = 29]) were investigated using ribotyping, toxin gene detection, resistance testing, multiple-locus variable-number tandem repeat analysis (MLVA), and whole genome sequencing (WGS). Results: Among chicken isolates, the most common RTs were RT103 (6/30), RT025 (5/30) and RT070 (4/30). Within soil samples, RT025 and RT056 were most common (3/21 each). In contrast, the non-toxigenic RT084 was most frequently found in human isolates (4/29). Toxin genes were detected in only 19/29 human isolates. Susceptibility testing showed no resistance against metronidazole and vancomycin, and resistance against macrolides and rifampicin was scarce (3/80 and 2/80, respectively); however, 26/80 isolates showed moxifloxacin resistance. MLVA and WGS of strains with identical RTs stemming from different sources revealed clustering of RT025 and RT084 isolates from human und non-human samples. Conclusion: No "hypervirulent” strains were found. The detected clusters between human, chicken and soil isolates indicate ongoing transmission between humans and environmental sources and might point towards a zoonotic potential.
DOI of the first publication: 10.1016/j.ijid.2020.04.026
Link to this record: urn:nbn:de:bsz:291--ds-324478
ISSN: 1878-3511
Date of registration: 5-Oct-2020
Description of the related object: Appendix A. Supplementary data
Related object:
Faculty: M - Medizinische Fakultät
Department: M - Infektionsmedizin
Professorship: M - Prof. Dr. Dr. Sören Becker
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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