Please use this identifier to cite or link to this item:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-30936
Title: | Reversible immobilization of a protein to a gold surface through multiple host-guest interactions |
Author(s): | Schwarz, Dennis H. Elgaher, Walid A. M. Hollemeyer, Klaus Hirsch, Anna Wenz, Gerhard |
Language: | English |
Title: | Journal of materials chemistry |
Volume: | 7 |
Issue: | 40 |
Startpage: | 6148 |
Endpage: | 6155 |
Publisher/Platform: | RSC |
Year of Publication: | 2019 |
Publikation type: | Journal Article |
Abstract: | Monolayers were formed by specific interactions between adamantylated proteins (transferrin, lysozyme) and a β-cyclodextrin (β-CD) monolayer on a gold surface. Very high stabilities could be reached by multiple interactions of 3-6 adamantyl moieties linked through triethylene glycol spacers to the protein with β-CD rings attached to the surface. Furthermore, bound proteins could be completely removed from the surface through competitive binding of an excess of free adamantane. Regenerable protein sensor chips can be constructed by using this supramolecular toolbox. Attached proteins are still recognized by specific antibodies, which was attributed to a loose packing of the protein molecules at the β-CD monolayer. |
DOI of the first publication: | 10.1039/c9tb00560a |
URL of the first publication: | https://pubs.rsc.org/en/content/articlelanding/2019/tb/c9tb00560a#!divAbstract |
Link to this record: | hdl:20.500.11880/29150 http://dx.doi.org/10.22028/D291-30936 |
ISSN: | 2050-7518 2050-750X |
Date of registration: | 14-May-2020 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Pharmazie |
Professorship: | NT - Prof. Dr. Anna Hirsch |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
There are no files associated with this item.
Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.