Please use this identifier to cite or link to this item: doi:10.22028/D291-27513
Title: Improved Chondrogenic Differentiation of rAAV SOX9-Modified Human MSCs Seeded in Fibrin-Polyurethane Scaffolds in a Hydrodynamic Environment
Author(s): Venkatesan, Jagadeesh K.
Gardner, Oliver
Rey-Rico, Ana
Eglin, David
Alini, Mauro
Stoddart, Martin J.
Cucchiarini, Magali
Madry, Henning
Language: English
Title: International Journal of Molecular Sciences
Volume: 19
Issue: 9
Publisher/Platform: MDPI
Year of Publication: 2018
Free key words: cartilage repair
fibrin-polyurethane scaffolds
DDC notations: 610 Medicine and health
Publikation type: Journal Article
Abstract: The repair of focal articular cartilage defects remains a problem. Combining gene therapy with tissue engineering approaches using bone marrow-derived mesenchymal stem cells (MSCs) may allow the development of improved options for cartilage repair. Here, we examined whether a three-dimensional fibrin-polyurethane scaffold provides a favorable environment for the effective chondrogenic differentiation of human MSCs (hMSCs) overexpressing the cartilage-specific SOX9 transcription factor via recombinant adeno-associated virus (rAAV) -mediated gene transfer cultured in a hydrodynamic environment in vitro. Sustained SOX9 expression was noted in the constructs for at least 21 days, the longest time point evaluated. Such spatially defined SOX9 overexpression enhanced proliferative, metabolic, and chondrogenic activities compared with control (reporter lacZ gene transfer) treatment. Of further note, administration of the SOX9 vector was also capable of delaying premature hypertrophic and osteogenic differentiation in the constructs. This enhancement of chondrogenesis by spatially defined overexpression of human SOX9 demonstrate the potential benefits of using rAAV-modified hMSCs seeded in fibrin-polyurethane scaffolds as a promising approach for implantation in focal cartilage lesions to improve cartilage repair.
DOI of the first publication: 10.3390/ijms19092635
Link to this record: urn:nbn:de:bsz:291--ds-275130
ISSN: 1422-0067
Date of registration: 19-Mar-2020
Faculty: M - Medizinische Fakultät
Department: M - Orthopädie
Professorship: M - Prof. Dr. Henning Madry
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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