Please use this identifier to cite or link to this item:
doi:10.22028/D291-27488
Title: | Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori |
Author(s): | Filomena, Angela Pessler, Frank Akmatov, Manas K. Krause, Gérard Duffy, Darragh Gärtner, Barbara Gerhard, Markus Albert, Matthew L. Joos, Thomas O. Schneiderhan-Marra, Nicole |
Language: | English |
Title: | High-Throughput |
Volume: | 6 |
Issue: | 4 |
Publisher/Platform: | MDPI |
Year of Publication: | 2017 |
Free key words: | multiplex serotest |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | The spread of infectious diseases and vaccination history are common subjects of epidemiological and immunological research studies. Multiplexed serological assays are useful tools for assessing both current and previous infections as well as vaccination efficacy. We developed a serological multi-pathogen assay for hepatitis A, B and C virus, cytomegalovirus (CMV), Toxoplasma gondii, and Helicobacter pylori using a bead-based multiplex assay format. The multi-pathogen assay consisting of 15 antigens was utilized for the analysis of the serological response in elderly individuals of an influenza vaccination study (n = 34). The technical assay validation revealed a mean intra-assay precision of coefficient of variation (CV) = 3.2 ± 1.5% and a mean inter-assay precision of CV = 8.2 ± 5.3% across all 15 antigens and all tested samples, indicating a robust test system. Furthermore, the assay shows high sensitivities (ranging between 94% and 100%) and specificities (ranging between 93% and 100%) for the different pathogens. The highest seroprevalence rates in our cohort were observed for hepatitis A virus (HAV; 73.5%), followed by CMV (70.6%), T. gondii (67.6%) and H. pylori (32.4%). Seroprevalences for hepatitis B virus (HBV, 8.8%) and hepatitis C virus (HCV, 0%) were low. The seroprevalences observed in our study were similar to those from other population-based studies in Germany. In summary, we conclude that our multiplex serological assay represents a suitable tool for epidemiological studies. |
DOI of the first publication: | 10.3390/ht6040014 |
Link to this record: | urn:nbn:de:bsz:291--ds-274887 hdl:20.500.11880/28596 http://dx.doi.org/10.22028/D291-27488 |
ISSN: | 2571-5135 |
Date of registration: | 17-Jan-2020 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Infektionsmedizin |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
---|---|---|---|---|
high-throughput-06-00014-v3.pdf | 720,1 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License