Please use this identifier to cite or link to this item:
doi:10.22028/D291-27473
Title: | An Optimized Facile Procedure to Synthesize and Purify Allicin |
Author(s): | Albrecht, Frank Leontiev, Roman Jacob, Claus Slusarenko, Alan J. |
Language: | English |
Title: | Molecules |
Volume: | 22 |
Issue: | 5 |
Publisher/Platform: | MDPI |
Year of Publication: | 2017 |
DDC notations: | 540 Chemistry |
Publikation type: | Journal Article |
Abstract: | Allicin is a reactive sulfur species (RSS) and defence substance from garlic (Allium sativum L.). The compound is a broad-spectrum antibiotic that is also effective against multiple drug resistant (MDR) strains. A detailed protocol for allicin synthesis based on diallyl-disulfide (DADS) oxidation by H2O2 using acetic acid as a catalyst was published in 2001 by Lawson and Wang. Here we report on improvements to this basic method, clarify the mechanism of the reaction and show that it is zero-order with respect to DADS and first-order with respect to the concentration of H2O2. The progress of allicin synthesis and the reaction mechanism were analyzsd by high-performance liquid chromatography (HPLC) and the identity and purity of the products was verified with LC-MS and 1H-NMR. We were able to obtain allicin of high purity (>98%) and >91% yield, with standard equipment available in any reasonable biological laboratory. This protocol will enable researchers to prepare and work with easily and cheaply prepared allicin of high quality. |
DOI of the first publication: | 10.3390/molecules22050770 |
Link to this record: | urn:nbn:de:bsz:291--ds-274731 hdl:20.500.11880/28570 http://dx.doi.org/10.22028/D291-27473 |
ISSN: | 1420-3049 |
Date of registration: | 13-Jan-2020 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Pharmazie |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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molecules-22-00770-v2.pdf | 2,43 MB | Adobe PDF | View/Open |
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