Please use this identifier to cite or link to this item: doi:10.22028/D291-27473
Title: An Optimized Facile Procedure to Synthesize and Purify Allicin
Author(s): Albrecht, Frank
Leontiev, Roman
Jacob, Claus
Slusarenko, Alan J.
Language: English
Title: Molecules
Volume: 22
Issue: 5
Publisher/Platform: MDPI
Year of Publication: 2017
DDC notations: 540 Chemistry
Publikation type: Journal Article
Abstract: Allicin is a reactive sulfur species (RSS) and defence substance from garlic (Allium sativum L.). The compound is a broad-spectrum antibiotic that is also effective against multiple drug resistant (MDR) strains. A detailed protocol for allicin synthesis based on diallyl-disulfide (DADS) oxidation by H2O2 using acetic acid as a catalyst was published in 2001 by Lawson and Wang. Here we report on improvements to this basic method, clarify the mechanism of the reaction and show that it is zero-order with respect to DADS and first-order with respect to the concentration of H2O2. The progress of allicin synthesis and the reaction mechanism were analyzsd by high-performance liquid chromatography (HPLC) and the identity and purity of the products was verified with LC-MS and 1H-NMR. We were able to obtain allicin of high purity (>98%) and >91% yield, with standard equipment available in any reasonable biological laboratory. This protocol will enable researchers to prepare and work with easily and cheaply prepared allicin of high quality.
DOI of the first publication: 10.3390/molecules22050770
Link to this record: urn:nbn:de:bsz:291--ds-274731
ISSN: 1420-3049
Date of registration: 13-Jan-2020
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
molecules-22-00770-v2.pdf2,43 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons