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doi:10.22028/D291-29606
Title: | Ciprofloxacin-loaded PLGA nanoparticles against cystic fibrosis P. aeruginosa lung infections |
Author(s): | Günday Türeli, Nazende Torge, Afra Juntke, Jenny Schwarz, Bianca C. Schneider-Daum, Nicole Türeli, Akif Emre Lehr, Claus-Michael Schneider, Marc |
Language: | English |
Title: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences |
Volume: | 117 |
Startpage: | 363 |
Endpage: | 371 |
Publisher/Platform: | Elsevier |
Year of Publication: | 2017 |
Publikation type: | Journal Article |
Abstract: | Current pulmonary treatments against Pseudomonas aeruginosa infections in cystic fibrosis (CF) lung suffer from deactivation of the drug and immobilization in thick and viscous biofilm/mucus blend, along with the general antibiotic resistance. Administration of nanoparticles (NPs) with high antibiotic load capable of penetrating the tight mesh of biofilm/mucus can be an advent to overcome the treatment bottlenecks. Biodegradable and biocompatible polymer nanoparticles efficiently loaded with ciprofloxacin complex offer a solution for emerging treatment strategies. NPs were prepared under controlled conditions by utilizing MicroJet Reactor (MJR) to yield a particle size of 190.4±28.6nm with 0.089 PDI. Encapsulation efficiency of the drug was 79% resulting in a loading of 14%. Release was determined to be controlled and medium-independent in PBS, PBS+0.2% Tween 80 and simulated lung fluid. Cytotoxicity assays with Calu-3 cells and CF bronchial epithelial cells (CFBE41o-) indicated that complex-loaded PLGA NPs were non-toxic at concentrations ≫ MICcipro against lab strains of the bacteria. Antibacterial activity tests revealed enhanced activity when applied as nanoparticles. NPs' colloidal stability in mucus was proven. Notably, a decrease in mucus turbidity was observed upon incubation with NPs. Herewith, ciprofloxacin complex-loaded PLGA NPs are introduced as promising pulmonary nano drug delivery systems against P.aeruginosa infections in CF lung. |
DOI of the first publication: | 10.1016/j.ejpb.2017.04.032 |
URL of the first publication: | https://www.sciencedirect.com/science/article/abs/pii/S0939641116309924?via%3Dihub |
Link to this record: | hdl:20.500.11880/27991 http://dx.doi.org/10.22028/D291-29606 |
ISSN: | 1879-0720 0928-0987 |
Date of registration: | 1-Oct-2019 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Pharmazie |
Professorship: | NT - Prof. Dr. Marc Schneider |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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