Please use this identifier to cite or link to this item:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-29523
Title: | Genomic integrity of ground-state pluripotency |
Author(s): | Jafari, Narges Giehr, Pascal Hesaraki, Mahdi Baas, Roy de Graaf, Petra Timmers, H. T. Marc Walter, Jörn Erik Baharvand, Hossein Totonchi, Mehdi |
Language: | English |
Title: | Journal of cellular biochemistry |
Volume: | 119 |
Issue: | 12 |
Startpage: | 9781 |
Endpage: | 9789 |
Publisher/Platform: | Wiley-Blackwell - STM |
Year of Publication: | 2018 |
Publikation type: | Journal Article |
Abstract: | Pluripotent cells appear to be in a transient state during early development. These cells have the capability to transition into embryonic stem cells (ESCs). It has been reported that mouse pluripotent cells cultivated in chemically defined media sustain the ground state of pluripotency. Because the epigenetic pattern of pluripotent cells reflects their environment, culture under different conditions causes epigenetic changes, which could lead to genomic instability. This study focused on the DNA methylation pattern of repetitive elements (REs) and their activation levels under two ground-state conditions and assessed the genomic integrity of ESCs. We measured the methylation and expression level of REs in different media. The results indicated that although the ground-state conditions show higher REs activity, they did not lead to DNA damage; therefore, the level of genomic instability is lower under the ground-state compared with the conventional condition. Our results indicated that when choosing an optimum condition, different features of the condition must be considered to have epigenetically and genomically stable stem cells. |
DOI of the first publication: | 10.1002/jcb.27296 |
Link to this record: | hdl:20.500.11880/27911 http://dx.doi.org/10.22028/D291-29523 |
ISSN: | 1097-4644 1547-9366 0091-7419 0730-2312 |
Date of registration: | 26-Sep-2019 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Biowissenschaften |
Professorship: | NT - Prof. Dr. Jörn Walter |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
There are no files associated with this item.
Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.