Please use this identifier to cite or link to this item: doi:10.22028/D291-29523
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Title: Genomic integrity of ground-state pluripotency
Author(s): Jafari, Narges
Giehr, Pascal
Hesaraki, Mahdi
Baas, Roy
de Graaf, Petra
Timmers, H. T. Marc
Walter, Jörn Erik
Baharvand, Hossein
Totonchi, Mehdi
Language: English
Title: Journal of cellular biochemistry
Volume: 119
Issue: 12
Startpage: 9781
Endpage: 9789
Publisher/Platform: Wiley-Blackwell - STM
Year of Publication: 2018
Publikation type: Journal Article
Abstract: Pluripotent cells appear to be in a transient state during early development. These cells have the capability to transition into embryonic stem cells (ESCs). It has been reported that mouse pluripotent cells cultivated in chemically defined media sustain the ground state of pluripotency. Because the epigenetic pattern of pluripotent cells reflects their environment, culture under different conditions causes epigenetic changes, which could lead to genomic instability. This study focused on the DNA methylation pattern of repetitive elements (REs) and their activation levels under two ground-state conditions and assessed the genomic integrity of ESCs. We measured the methylation and expression level of REs in different media. The results indicated that although the ground-state conditions show higher REs activity, they did not lead to DNA damage; therefore, the level of genomic instability is lower under the ground-state compared with the conventional condition. Our results indicated that when choosing an optimum condition, different features of the condition must be considered to have epigenetically and genomically stable stem cells.
DOI of the first publication: 10.1002/jcb.27296
Link to this record: hdl:20.500.11880/27911
ISSN: 1097-4644
Date of registration: 26-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Jörn Walter
Collections:Die Universitätsbibliographie

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