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doi:10.22028/D291-28892
Title: | Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code |
Author(s): | Hauf, Matthias Richter, Florian Schneider, Tobias Faidt, Thomas Martins, Berta M. Baumann, Tobias Durkin, Patrick Dobbek, Holger Jacobs, Karin Möglich, Andreas Budisa, Nediljko |
Language: | English |
Title: | Chembiochem |
Volume: | 18 |
Issue: | 18 |
Startpage: | 1819 |
Endpage: | 1823 |
Publisher/Platform: | Wiley-VCH |
Year of Publication: | 2017 |
Publikation type: | Journal Article |
Abstract: | Marine mussels exhibit potent underwater adhesion abilities under hostile conditions by employing 3,4-dihydroxyphenylalanine (DOPA)-rich mussel adhesive proteins (MAPs). However, their recombinant production is a major biotechnological challenge. Herein, a novel strategy based on genetic code expansion has been developed by engineering efficient aminoacyl-transfer RNA synthetases (aaRSs) for the photocaged noncanonical amino acid ortho-nitrobenzyl DOPA (ONB-DOPA). The engineered ONB-DOPARS enables in vivo production of MAP type 5 site-specifically equipped with multiple instances of ONB-DOPA to yield photocaged, spatiotemporally controlled underwater adhesives. Upon exposure to UV light, these proteins feature elevated wet adhesion properties. This concept offers new perspectives for the production of recombinant bioadhesives. |
DOI of the first publication: | 10.1002/cbic.201700327 |
Link to this record: | hdl:20.500.11880/27771 http://dx.doi.org/10.22028/D291-28892 |
ISSN: | 14394227 |
Date of registration: | 13-Sep-2019 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Physik |
Professorship: | NT - Prof. Dr. Karin Jacobs |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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