Please use this identifier to cite or link to this item: doi:10.22028/D291-28892
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Title: Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code
Author(s): Hauf, Matthias
Richter, Florian
Schneider, Tobias
Faidt, Thomas
Martins, Berta M.
Baumann, Tobias
Durkin, Patrick
Dobbek, Holger
Jacobs, Karin
Möglich, Andreas
Budisa, Nediljko
Language: English
Title: Chembiochem
Volume: 18
Issue: 18
Startpage: 1819
Endpage: 1823
Publisher/Platform: Wiley-VCH
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Marine mussels exhibit potent underwater adhesion abilities under hostile conditions by employing 3,4-dihydroxyphenylalanine (DOPA)-rich mussel adhesive proteins (MAPs). However, their recombinant production is a major biotechnological challenge. Herein, a novel strategy based on genetic code expansion has been developed by engineering efficient aminoacyl-transfer RNA synthetases (aaRSs) for the photocaged noncanonical amino acid ortho-nitrobenzyl DOPA (ONB-DOPA). The engineered ONB-DOPARS enables in vivo production of MAP type 5 site-specifically equipped with multiple instances of ONB-DOPA to yield photocaged, spatiotemporally controlled underwater adhesives. Upon exposure to UV light, these proteins feature elevated wet adhesion properties. This concept offers new perspectives for the production of recombinant bioadhesives.
DOI of the first publication: 10.1002/cbic.201700327
Link to this record: hdl:20.500.11880/27771
ISSN: 14394227
Date of registration: 13-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Karin Jacobs
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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