Please use this identifier to cite or link to this item:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-28877
Title: | Dynamic Assembly of Class II Hydrophobins from T. reesei at the Air-Water Interface |
Author(s): | Hähl, Hendrik Griffo, Alessandra Safaridehkohneh, Neda Heppe, Jonas Backes, Sebastian Lienemann, Michael Linder, Markus B. Santen, Ludger Laaksonen, Päivi Jacobs, Karin |
Language: | English |
Title: | Langmuir : the ACS journal of surfaces and colloids |
Volume: | 35 |
Issue: | 28 |
Startpage: | 9202 |
Endpage: | 9212 |
Publisher/Platform: | ACS |
Year of Publication: | 2019 |
Publikation type: | Journal Article |
Abstract: | Class II hydrophobins are amphiphilic proteins produced by filamentous fungi. One of their typical features is the tendency to accumulate at the interface between an aqueous phase and a hydrophobic phase, such as the air-water interface. The kinetics of the interfacial self-assembly of wild-type hydrophobins HFBI and HFBII and some of their engineered variants at the air-water interface were measured by monitoring the accumulated mass at the interface via nondestructive ellipsometry measurements. The resulting mass vs time curves revealed unusual kinetics for a monolayer formation that did not follow a typical Langmuir-type of behavior but had a rather coverage-independent rate instead. Typically, the full surface coverage was obtained at masses corresponding to a monolayer. The formation of multilayers was not observed. Atomic force microscopy revealed formation and growth of non-fusing protein clusters at the interface. The mechanism of the adsorption was studied by varying the structure or charges of the protein or the ionic strength of the subphase, revealing that the lateral interactions between the hydrophobins play a role in their interfacial assembly. Additionally, a theoretical model was introduced to identify the underlying mechanism of the unconventional adsorption kinetics. |
DOI of the first publication: | 10.1021/acs.langmuir.9b01078 |
URL of the first publication: | https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.9b01078 |
Link to this record: | hdl:20.500.11880/27762 http://dx.doi.org/10.22028/D291-28877 |
ISSN: | 1520-5827 0743-7463 |
Date of registration: | 13-Sep-2019 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Physik |
Professorship: | NT - Prof. Dr. Karin Jacobs |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
There are no files associated with this item.
Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.