Please use this identifier to cite or link to this item: doi:10.22028/D291-28877
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Title: Dynamic Assembly of Class II Hydrophobins from T. reesei at the Air-Water Interface
Author(s): Hähl, Hendrik
Griffo, Alessandra
Safaridehkohneh, Neda
Heppe, Jonas
Backes, Sebastian
Lienemann, Michael
Linder, Markus B.
Santen, Ludger
Laaksonen, Päivi
Jacobs, Karin
Language: English
Title: Langmuir
Volume: 35
Issue: 28
Startpage: 9202
Endpage: 9212
Publisher/Platform: American Chemical Society (ACS)
Year of Publication: 2019
Publikation type: Journal Article
Abstract: Class II hydrophobins are amphiphilic proteins produced by filamentous fungi. One of their typical features is the tendency to accumulate at the interface between an aqueous phase and a hydrophobic phase, such as the air-water interface. The kinetics of the interfacial self-assembly of wild-type hydrophobins HFBI and HFBII and some of their engineered variants at the air-water interface were measured by monitoring the accumulated mass at the interface via nondestructive ellipsometry measurements. The resulting mass vs time curves revealed unusual kinetics for a monolayer formation that did not follow a typical Langmuir-type of behavior but had a rather coverage-independent rate instead. Typically, the full surface coverage was obtained at masses corresponding to a monolayer. The formation of multilayers was not observed. Atomic force microscopy revealed formation and growth of non-fusing protein clusters at the interface. The mechanism of the adsorption was studied by varying the structure or charges of the protein or the ionic strength of the subphase, revealing that the lateral interactions between the hydrophobins play a role in their interfacial assembly. Additionally, a theoretical model was introduced to identify the underlying mechanism of the unconventional adsorption kinetics.
DOI of the first publication: 10.1021/acs.langmuir.9b01078
Link to this record: hdl:20.500.11880/27762
http://dx.doi.org/10.22028/D291-28877
ISSN: 0743-7463
1520-5827
Date of registration: 13-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Collections:UniBib – Die Universitätsbibliographie

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