Please use this identifier to cite or link to this item:
doi:10.22028/D291-27639
Title: | Development, Applicability, and Comparison of Bioassays for Metabolism of (New) Psychoactive Substances |
Author(s): | Richter, Lilian Helga Johanne |
Language: | English |
Year of Publication: | 2018 |
Place of publication: | Saarbrücken |
DDC notations: | 500 Science |
Publikation type: | Dissertation |
Abstract: | In the presented dissertation, different in vitro models were investigated and compared as surrogate for human in vivo metabolism studies to identify main urinary excretion products for developing toxicological urine screening approaches for new psychoactive substances (NPS). Assays using human liver preparations, which covered all main metabolic phase I and II steps were developed. Human hepatic cell lines HepG2 and HepaRG were tested as an alternative in vitro model. However, cell lines have the disadvantage of requiring special equipment for cell culture handling. Therefore, pS9 fraction was identified as most cost-efficient and easiest to handle in vitro model, still sufficient enough for developing toxicological urine screenings for NPS, even in comparison to primary human hepatocytes. |
Link to this record: | urn:nbn:de:bsz:291--ds-276396 hdl:20.500.11880/27294 http://dx.doi.org/10.22028/D291-27639 |
Advisor: | Maurer, Hans Hilarius |
Date of oral examination: | 14-Dec-2018 |
Date of registration: | 15-Jan-2019 |
Faculty: | NT - Naturwissenschaftlich- Technische Fakultät |
Department: | NT - Pharmazie |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
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Dissertation_Richter_LHJ_Druck_SULB.pdf | 4,79 MB | Adobe PDF | View/Open |
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