Please use this identifier to cite or link to this item:
doi:10.22028/D291-21400
Title: | CAPS1 regulates catecholamine loading of large dense-core vesicles |
Author(s): | Speidel, Dina Bruederle, Cathrin E. Enk, Carsten Voets, Thomas Varoqueaux, Frederique Reim, Kerstin Becherer, Ute Fornai, Francesco Ruggieri, Stefano Holighaus, Yvonne Weihe, Eberhard Bruns, Dieter Brose, Nils Rettig, Jens |
Language: | German |
Year of Publication: | 2005 |
OPUS Source: | Neuron. - 46. 2005, 1, S. 75-88 |
DDC notations: | 570 Life sciences, biology |
Publikation type: | Journal Article |
Abstract: | CAPS1 is thought to play an essential role in mediating exocytosis from large dense-core vesicles (LDCVs). We generated CAPS1-deficient (KO) mice and studied exocytosis in a model system for Ca2+-dependent LDCV secretion, the adrenal chromaffin cell. Adult heterozygous CAPS1 KO cells display a gene dosage-dependent decrease of CAPS1 expression and a concomitant reduction in the number of docked vesicles and secretion. Embryonic homozygous CAPS1 KO cells show a strong reduction in the frequency of amperometrically detectable release events of transmitter-filled vesicles, while the total number of fusing vesicles, as judged by capacitance recordings or total internal reflection microscopy, remains unchanged. We conclude that CAPS1 is required for an essential step in the uptake or storage of catecholamines in LDCVs. |
Link to this record: | urn:nbn:de:bsz:291-scidok-32898 hdl:20.500.11880/21456 http://dx.doi.org/10.22028/D291-21400 |
Date of registration: | 2-May-2011 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Physiologie |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
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