Please use this identifier to cite or link to this item: doi:10.22028/D291-21400
Title: CAPS1 regulates catecholamine loading of large dense-core vesicles
Author(s): Speidel, Dina
Bruederle, Cathrin E.
Enk, Carsten
Voets, Thomas
Varoqueaux, Frederique
Reim, Kerstin
Becherer, Ute
Fornai, Francesco
Ruggieri, Stefano
Holighaus, Yvonne
Weihe, Eberhard
Bruns, Dieter
Brose, Nils
Rettig, Jens
Language: German
Year of Publication: 2005
OPUS Source: Neuron. - 46. 2005, 1, S. 75-88
DDC notations: 570 Life sciences, biology
Publikation type: Journal Article
Abstract: CAPS1 is thought to play an essential role in mediating exocytosis from large dense-core vesicles (LDCVs). We generated CAPS1-deficient (KO) mice and studied exocytosis in a model system for Ca2+-dependent LDCV secretion, the adrenal chromaffin cell. Adult heterozygous CAPS1 KO cells display a gene dosage-dependent decrease of CAPS1 expression and a concomitant reduction in the number of docked vesicles and secretion. Embryonic homozygous CAPS1 KO cells show a strong reduction in the frequency of amperometrically detectable release events of transmitter-filled vesicles, while the total number of fusing vesicles, as judged by capacitance recordings or total internal reflection microscopy, remains unchanged. We conclude that CAPS1 is required for an essential step in the uptake or storage of catecholamines in LDCVs.
Link to this record: urn:nbn:de:bsz:291-scidok-32898
Date of registration: 2-May-2011
Faculty: M - Medizinische Fakultät
Department: M - Physiologie
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
File Description SizeFormat 
Speidel.pdf677,75 kBAdobe PDFView/Open

Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.