Please use this identifier to cite or link to this item:
doi:10.22028/D291-21005
Title: | Prospective study on the expression of cancer testis genes and antibody responses in 100 consecutive patients with primary breast cancer |
Author(s): | Mischo, Axel Kubuschok, Boris Ertan, Kubilay Preuss, Klaus-Dieter Romeike, Bernd F. M. Regitz, Evi Schormann, Claudia de Bruijn, Diederik Wadle, Andreas Neumann, Frank Schmidt, Werner Renner, Christoph Pfreundschuh, Michael |
Language: | German |
Year of Publication: | 2006 |
OPUS Source: | International journal of cancer. - 118. 2006, 3, S. 696-703 |
DDC notations: | 610 Medicine and health |
Publikation type: | Journal Article |
Abstract: | To determine the expression of cancer testis (CT) genes and antibody responses in a nonselected population of patients with primary breast cancer, we investigated the composite expression of 11 CT genes by RT-PCR in fresh biopsies of 100 consecutive cases of primary breast carcinoma and by immunohistology in selected RT-PCR-positive cases. Antibody responses against 7 CT antigens were analyzed using recombinant antigen expression on yeast surface. In 98 evaluable cases, SCP-1 and SSX-4 were expressed most frequently (both 65%), followed by HOM-TES-85/CT-8 (47%), GAGE (26%), SSX-1 (20%), NY-ESO-1 (13%), MAGE-3 (11%), SSX-2 (8%), CT-10 (7%), MAGE-4 (4%) and CT-7 (1%). One CT gene was expressed by 90% of the cases; 79% expressed > or =2, 48% > or =3, 29% > or =4, 12% > or =5, 6% > or =6, 3% > or =7, 2% > or =8 and one case coexpressed 9 antigens. Of 100 serum samples screened for CT antigen-specific antibodies, antibodies against NY-ESO-1 were detected in 4 patients, against SCP-1 in 6 patients and against SSX-2 in 1 patient, while no antibodies were detected against MAGE-3, CT-7 and CT-10. Expression of CT genes or antibody responses was not correlated with clinical parameters (menopausal status, tumor size, nodal involvement, grading, histology and estrogen receptor status) or the demonstration of CT gene expression at the protein level, by immunohistology. Our results show that breast carcinomas are among the tumors with the most frequent expression of CT antigens, rendering many patients potential candidates for vaccine trials. |
Link to this record: | urn:nbn:de:bsz:291-scidok-15379 hdl:20.500.11880/21061 http://dx.doi.org/10.22028/D291-21005 |
Date of registration: | 15-May-2008 |
Faculty: | M - Medizinische Fakultät |
Department: | M - Innere Medizin M - Frauenheilkunde M - Pathologie |
Collections: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Files for this record:
File | Description | Size | Format | |
---|---|---|---|---|
ct_antigens_mamma_ca.pdf | 555,36 kB | Adobe PDF | View/Open |
Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.