MALDI-TOF mass spectrometry combined with machine learning algorithms to identify protein profiles related to malaria infection in human sera from Côte d’Ivoire

Abstract

Background In sub-Saharan Africa, Plasmodium falciparum is the most prevalent species of malaria parasites. In endemic areas, malaria is mainly diagnosed using microscopy or rapid diagnostic tests (RDTs), which have limited sensitivity, and microscopic expertise is waning in non-endemic regions. Matrix-assisted laser desorption/ionization timeof-fight (MALDI-TOF) mass spectrometry (MS) is nowadays the standard method in routine microbiology laboratories for bacteria and fungi identifcation in high-income countries, but is rarely used for parasite detection. This study aims to employ MALDI-TOF MS for identifying malaria by distinguishing P. falciparum-positive from P. falciparum-negative sera. Methods Sera were obtained from 282 blood samples collected from non-febrile, asymptomatic people aged 5 to 58 years in southern Côte d’Ivoire. Infectious status and parasitaemia were determined by both RDTs and microscopy, followed by a categorization into two groups (P. falciparum-positive and P. falciparum-negative samples). MALDI-TOF MS analysis was carried out by generating protein spectra profles from 131 Plasmodium-positive and 94 Plasmodium-negative sera as the training set. Machine learning (ML) algorithms were employed for distinguishing P. falciparum-positive from P. falciparum-negative samples. Subsequently, a subset of 57 sera (42 P. falciparum-positive and 15 P. falciparum-negative) was used as the validation set to evaluate the best two of the four models trained. Results MALDI-TOF MS was able to generate good-quality spectra from both P. falciparum-positive and P. falciparumnegative serum samples. High similarities between the protein spectra profles did not allow for distinguishing the two groups using principal component analysis (PCA). When four supervised ML algorithms were tested by tenfold cross-validation, P. falciparum-positive sera were discriminated against P. falciparum-negative sera with a global accuracy ranging from 73.28% to 81.30%, while sensitivity ranged from 70.23% to 83.97%. The independent test performed with a subset of 57 serum samples showed accuracies of 85.96% and 89.47%, and sensitivities of 90.48% and 92.86%, respectively, for LightGBM and RF. Conclusion MALDI-TOF MS combined with ML might be applied for detection of protein profles related to P. falciparum malaria infection in human serum samples. Additional research is warranted for further optimization such as specifc biomarkers detection or using other ML models.