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Titel: Effect of MiRNA 204-5P Mimics and Lipopolysaccharide-Induced Inflammation on Transcription Factor Levels, Cell Maintenance, and Retinoic Acid Signaling in Primary Limbal Epithelial Cells
VerfasserIn: Amini, Maryam
Stachon, Tanja
Hsu, Shao-Lun
Li, Zhen
Chai, Ning
Fries, Fabian N.
Seitz, Berthold
Kundu, Swarnali
Suiwal, Shweta
Szentmáry, Nóra
Sprache: Englisch
Titel: International Journal of Molecular Sciences
Bandnummer: 26
Heft: 8
Verlag/Plattform: MDPI
Erscheinungsjahr: 2025
Freie Schlagwörter: miR-204-5p
limbal epithelial cells
lipopolysaccharide
inflammation
miRNA mimic
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: MicroRNA-204-5p (miR-204-5p) is a critical regulator of differentiation, structural maintenance, and inflammation in limbal epithelial cells (LECs). This study examined the role of miR-204-5p in modulating the gene expression related to transcription factors, cell structure, extracellular matrix remodeling, and retinoic acid signaling under normal and lipopolysaccharide (LPS)-induced inflammatory conditions. Using qPCR, we analyzed the mRNA levels of FOSL2, FOXC1, Meis2, PPARγ, ABCG2, PTGES2, IL-1β, IL-6, KRT3, KRT12, MMP2, MMP9, RARA, RARB, RXRA, RXRB, CRABP2, RBP1, RDH10, ADH7, ADH1A1, FABP5, CYP1B1, and CYP26A1, while changes in protein levels were assessed via Western blot or ELISA. Our data revealed that the overexpression of miR-204-5p reduced the mRNA levels of FOXC1, KRT12, and RDH10 under normal and inflammatory conditions (p ≤ 0.039). Additionally, it decreased FOSL2 and RXRA mRNA under normal conditions (p = 0.006, p = 0.011) and KRT3 and FABP5 mRNA under inflammatory conditions (p = 0.010, p = 0.001). The IL-6 mRNA expression was significantly increased following the LPS treatment in cells overexpressing miR-204-5p (p = 0.029). A protein analysis revealed significant reductions in FOXC1 and KRT3 in the miR-204-5p-transfected cells during LPS-induced inflammation (p = 0.020, p = 0.030). These findings suggest that miR-204-5p modulates genes critical to the differentiation, migration, and inflammatory response of LECs. The modulation of FOXC1 and KRT3 by miR-204-5p highlights these proteins as novel targets under inflammatory conditions.
DOI der Erstveröffentlichung: 10.3390/ijms26083809
URL der Erstveröffentlichung: https://doi.org/10.3390/ijms26083809
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-451272
hdl:20.500.11880/39952
http://dx.doi.org/10.22028/D291-45127
ISSN: 1422-0067
Datum des Eintrags: 29-Apr-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Anatomie und Zellbiologie
M - Augenheilkunde
Professur: M - Prof. Dr. Frank Schmitz
M - Prof. Dr. Berthold Seitz
M - Prof. Dr. med. Nóra Szentmáry
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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