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Titel: Decreased PAX6 and DSG1 Protein Expression in Corneal Epithelium of Patients with Epithelial Basal Membrane Dystrophy, Salzmann Nodular Degeneration, and Pterygium
VerfasserIn: Stachon, Tanja
Fries, Fabian N.
Li, Zhen
Daas, Loay
Nagy, Zoltán Zsolt
Seitz, Berthold
Szentmáry, Nóra
Sprache: Englisch
Titel: Journal of Clinical Medicine
Bandnummer: 14
Heft: 5
Verlag/Plattform: MDPI
Erscheinungsjahr: 2025
Freie Schlagwörter: epithelial basal membrane dystrophy
Salzmann’s nodular degeneration
pterygium
molecular markers
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumenttyp: Journalartikel / Zeitschriftenartikel
Abstract: Background/Objectives: Evaluation of stem cell, keratin, retinoic acid metabolism markers and non-coding micro-RNAs (miRNAs) in conjunctival and corneal samples of patients with epithelial basal membrane dystrophy (EBMD), Salzmann nodular degeneration (SND), pterygium and congenital aniridia (CA), to detect similarities and differences in their pathogenesis. Methods: Impression cytology (IC) samples and corneal epithelial samples (CEs) of patients with EBMD, SND, pterygium, congenital aniridia, and healthy control subjects have been analyzed. The IC samples were subjected to qPCR, and the epithelial samples were subjected to qPCR and WB. Limbal epithelial stem cell markers, keratins, retinoic acid metabolism markers, and miRNAs were analyzed. Results: In conjunctival IC samples, PAX6 mRNA expression was significantly lower in EBMD, SND, pterygium, and CA compared to healthy controls (p ≤ 0.02). KRT13 mRNA expression was significantly higher in EBMD, SND, and pterygium (p ≤ 0.018), and FABP5 was increased in pterygium samples (p = 0.007). MiRNA-138-5p was significantly higher in aniridia samples than in normal controls (p = 0.037). In corneal epithelial samples, PAX6 protein, DSG1 mRNA and protein, miRNA-138-5p, and miR-204-5p expression were significantly lower in EBMD, SND, and pterygium samples than in controls (p ≤ 0.02). ALDHA1 mRNA expression was significantly lower (p < 0.0001), and FABP5 mRNA expression was significantly higher (p = 0.014) in pterygium samples than in controls. Conclusions: PAX6, DSG1, miR-138-5p, and miR-204-5p expression is decreased in the corneal epithelium of epithelial basal membrane dystrophy, Salzmann nodular degeneration, and pterygium subjects. In addition, there is a dysregulation of markers of the retinoic acid signaling pathway, such as ADH1A1 and FABP5, in the corneal epithelium of pterygium subjects. These changes may offer therapeutic targets in the treatment of these ocular surface diseases.
DOI der Erstveröffentlichung: 10.3390/jcm14051456
URL der Erstveröffentlichung: https://doi.org/10.3390/jcm14051456
Link zu diesem Datensatz: urn:nbn:de:bsz:291--ds-446280
hdl:20.500.11880/39779
http://dx.doi.org/10.22028/D291-44628
ISSN: 2077-0383
Datum des Eintrags: 12-Mär-2025
Fakultät: M - Medizinische Fakultät
Fachrichtung: M - Augenheilkunde
Professur: M - Prof. Dr. Berthold Seitz
M - Prof. Dr. med. Nóra Szentmáry
Sammlung:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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